Recombinant human CRIPT protein
CRIPT, also known as cysteine-rich PDZ-binding protein, is involved in the cytoskeletal anchoring of DLG4 in excitatory synapses. Recombinant human CRIPT protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.
Product Specifications
Product Name Alternative
Cysteine-rich PDZ-binding protein, HSPC139
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
1 mg/mL (determined by Bradford assay)
Purity
> 95% by SDS-PAGE
Molecular Weight
13.7 kDa (124aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Saro D., et al. (2007) Biochemistry. 46:6340-6352; ; Zhang Q.-H., et al. (2000) Genome Res. 10:1546-1560
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 20% glycerol, 0.1M NaCl, 1mM DTT
Scientific Category
Cytoskeleton
NCBI Accession Number
NP_054890
Uniprot Accession Number
Q9P021
Species
Human
AA Sequence
MVCEKCE KKLGTVITPD TWKDGARNTT ESGGRKLNEN KALTSKKARF DPYGKNKFST CRICKSSVHQ PGSHYCQGCA YKKGICAMCG KKVLDTKNYK QTSV
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