Recombinant human DUSP18 protein
Dual specificity phosphatase 18, also known as DuSP18, is a member of the dual-specificity phosphatase (DSP) family, which catalyzes dephosphorylation of phosphotyrosine and phosphothreonine residues. DuSP18 is inhibited by iodoarectic acid and is activated by manganese ions. Along with having preferential enzymatic activity against phosphorylated tyrosine residues over threonine residues, DuSP18 also dephosphorylates p-nitrophenyl phosphate (pNPP) in vitro. This protein is widely expressed with highest levels in liver, brain, ovary and testis. Recombinant human DuSP18 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Specifications
Product Name Alternative
Dual specificity protein phosphatase 18, DSP18, DUSP20, Low molecular weight dual specificity phosphatase 20, LMW-DSP20, LMWDSP20
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
0.5 mg/mL (determined by Bradford assay)
Purity
> 95% by SDS-PAGE
Molecular Weight
23.6 kDa (212aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Jeong D G., et al. (2006) Acta Crystallogr. 62:582-588.; ; Aoki N., et al. (2001) J Biochem. 130:133-140.;
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 40% glycerol, 0.1mM PMSF, 1mM EDTA
Scientific Category
Enzymes & Proteases
NCBI Accession Number
NP_689724
Uniprot Accession Number
Q8NEJ0
Species
Human
AA Sequence
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