Recombinant human DUSP10 protein
DuSP10 (Dual specificity protein phosphatase 10) belongs to the protein-tyrosine phosphatase family. Dual specificity protein phosphatases (DuSPs) inactivate their target kinases by dephosphorylating both the phosphoserine/threonine and phosphotyrosine residues. They negatively regulate members of the MAPK superfamily (MAPK/ERK, SAPK/JNK, p38), which is associated with cellular proliferation and differentiation. DuSP10 has been shown to interact with MAPK14 and MAPK8. When expressed in mammalian cells, DuSP10 blocks the enzymatic activation of MAP kinases with the selectivity p38 approximately JNK/SAPK >> ERK. Recombinant human DuSP10 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Specifications
Specifications
Product Name Alternative
Dual specificity protein phosphatase 10, MKP-5, MKP5, Mitogen-activated protein kinase phosphatase 5, MAP kinase phosphatase 5
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE, Enzyme Activity
Concentration
0.25 mg/mL (determined by Bradford assay)
Purity
> 90% by SDS-PAGE
Bioactivity
Molecular Weight
40.4 kDa (359aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Theodosiou A, et al. (1999) Oncogene. 18 (50) :6981-8.; ; Jeong DG, et al. (2006) J Mol Biol. 360 (5) :946-55.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 2mM DTT, 50% glycerol, 200 mM NaCl
Scientific Category
Enzymes & Proteases
NCBI Accession Number
NP_009138
Uniprot Accession Number
Q9Y6W6
Species
Human
AA Sequence
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