Recombinant human DNA polymerase lambda/POLL protein

DNA polymerase lambda, also known as POLL, is a DNA polymerase involved in base excision repair (BER) . POLL is a low-fidelity polymerase which plays a role in both spontaneous and DNA damage-induced mutagenesis. In addition, POLL may play a role in the mutagenic bypass of T-T dimers. Pol lambda possesses terminal transferase and 5'-deoxyribose-5-phosphate lyase activity. These activities have been found to be coordinated by PCNA and replication protein A. Recombinant human POLL protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

DNA polymerase lambda, BETA-N, POL-KAPPA, Pol beta2, POLKAPPA

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.5 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Molecular Weight

36 kDa (320aa) confirmed by MALDI-TOF

Additionnal Information

POLL, ATGP1255-10 µg, ATGP1255-20 µg, ATGP1255-50 µg, ATGP1255-100 µg, ATGP1255-250 µg, ATGP1255-500 µg, ATGP1255-1 mg, ATGP1255-10, ATGP1255-20, ATGP1255-50, ATGP1255-100, ATGP1255-250, ATGP1255-500, ATGP1255-1

References & Citations

O Wang J., et al. (2001) Cancer Res. 61:5366-5369.; ; Zhang Y., et al. (2000) Nucleic Acids Res. 28:4147-4156.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 30% glycerol, 0.1M NaCl

Scientific Category

Epigenetics and Nuclear Signaling

NCBI Accession Number

AAH03548

Uniprot Accession Number

Q9UGP5

Species

Human

AA Sequence

MLMHHQKYLQ RFLGGKREKK QKEACSIPGI GKRMAEKIIE ILESGHLRKL DHISESVPVL ELFSNIWGAG TKTAQMWYQQ GFRSLEDIRS QASLTTQQAI GLKHYSDFLE RMPREEATEI EQTVQKAAQA FNSGLLCVAC GSYRRGKATC GDVDVLITHP DGRSHRGIFS RLLDSLRQEG FLTDDLVSQE ENGQQQKYLG VCRLPGPGRR HRRLDIIVVP YSEFACALLY FTGSAHFNRS MRALAKTKGM SLSEHALSTA VVRNTHGCKV GPGRVLPTPT EKDVFRLLGL PYREPAERDW