Recombinant human NAPG protein
NAPG, also known as gamma-SNAP, is a cytoplasmic protein that binds to a membrane receptor complex composed of VAMP, SNAP25 and syntaxin. It mediates the membrane binding of NSF, which is essential for membrane fusion reactions. An additional protein designated synaptophysin may regulate exocytosis by competing with SNAP25 and syntaxins for VAMP binding. Recombinant human NAPG protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.
Product Specifications
Product Name Alternative
Gamma-soluble NSF attachment protein, GAMMA-SNAP
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
1 mg/mL (determined by Bradford assay)
Purity
> 95% by SDS-PAGE
Molecular Weight
37.3 kDa (336aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Bennett M.K. et al. (1993) Cell 74: 863-873.; ; McMahon H.T. et al. (1995) J. Biol. Chem. 270: 2213-2217.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 0.1M NaCl,1mM DTT
Scientific Category
Neuroscience
NCBI Accession Number
NP_003817.1
Uniprot Accession Number
Q99747
Species
Human
AA Sequence
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