Recombinant human KARS protein

KARS, also known as Lysyl-tRNA synthetase, belongs to the class-II aminoacyl-tRNA synthetase family. It exists as both mitochondrial and cytoplasmic isoforms produced by alternative splicing, and thought to play a role in autoimmune diseases, such as polymyositis or dermatomyositis. This protein functions to catalyze the aminoacylation of tRNAs by their corresponding amino acids, thus linking amino acids with tRNA-contained nucleotide triplets. Recombinant human KARS protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Product Specifications

Product Name Alternative

Lysyl-tRNA synthetase, CMTRIB, KARS2, KIAA0070, KRS

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 95% by SDS-PAGE

Molecular Weight

63.7 kDa (558aa)

Additionnal Information

KARS, Lysyl-tRNA synthetase, CMTRIB, KARS2, KIAA0070, KRS, ATGP1166-10 µg, ATGP1166-20 µg, ATGP1166-50 µg, ATGP1166-100 µg, ATGP1166-250 µg, ATGP1166-500 µg, ATGP1166-1 mg, ATGP1166-10, ATGP1166-20, ATGP1166-50, ATGP1166-100, ATGP1166-250, ATGP1166-500, ATGP1166-1

References & Citations

Targoff I.N. et al. (1993) J. Clin. Invest. 91: 2556-2564.; ; Tolkunova E. et al. (2000) J. Biol. Chem. 275: 35063-35069.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 20% glycerol, 0.1M NaCl,1mM DTT

Scientific Category

Enzymes & Proteases

NCBI Accession Number

NP_005539.1

Uniprot Accession Number

Q15046

Species

Human

AA Sequence

GVGPEEE SVDPNQYYKI RSQAIHQLKV NGEDPYPHKF HVDISLTDFI QKYSHLQPGD HLTDITLKVA GRIHAKRASG GKLIFYDLRG EGVKLQVMAN SRNYKSEEEF IHINNKLRRG DIIGVQGNPG KTKKGELSII PYEITLLSPC LHMLPHLHFG LKDKETRYRQ RYLDLILNDF VRQKFIIRSK IITYIRSFLD ELGFLEIETP MMNIIPGGAV AKPFITYHNE LDMNLYMRIA PELYHKMLVV GGIDRVYEIG RQFRNEGIDL THNPEFTTCE FYMAYADYHD LMEITEKMVS GMVKHITGSY KVTYHPDGPE GQAYDVDFTP PFRRINMVEE LEKALGMKLP ETNLFETEET RKILDDICVA KAVECPPPRT TARLLDKLVG EFLEVTCINP TFICDHPQIM SPLAKWHRSK EGLTERFELF VMKKEICNAY TELNDPMRQR QLFEEQAKAK AAGDDEAMFI DENFCTALEY GLPPTAGWGM GIDRVAMFLT DSNNIKEVLL FPAMKPEDKK ENVATTDTLE STTVGTSV