Recombinant e.coli ppa protein

Inorganic pyrophosphatase (ppa) belongs to the Ppase family. This protein is an enzyme that catalyzes the conversion of one molecule of pyrophosphate to two phosphate ions. This is a highly exergonic reaction, and therefore can be coupled to unfavorable biochemical transformations in order to drive these transformations to completion. The functionality of this enzyme plays a critical role in lipid metabolism (including lipid synthesis and degradation), calcium absorption and bone formation, and DNA synthesis, as well as other biochemical transformations. Recombinant E. coli ppa protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Inorganic pyrophosphatase, ECK4222, JW4185

Expression System

E.coli

Antigen Species

E.coli

Tag

His-Tag

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 95% by SDS-PAGE

Molecular Weight

21.9 kDa (196aa) confirmed by MALDI-TOF

Additionnal Information

Ppa, inorganic pyrophosphatase, ECK4222, JW4185, ATGP1132-10 µg, ATGP1132-20 µg, ATGP1132-50 µg, ATGP1132-100 µg, ATGP1132-250 µg, ATGP1132-500 µg, ATGP1132-1 mg, ATGP1132-10, ATGP1132-20, ATGP1132-50, ATGP1132-100, ATGP1132-250, ATGP1132-500, ATGP1132-1

References & Citations

Harold FM., et al (1966) Bacteriol Rev 30 (4) : 772-94.; ; Carman GM., et al (2006) Trends Biochem. Sci. 31 (12) : 694-9.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol, 50 mM NaCl

Scientific Category

Signal Transduction

NCBI Accession Number

NP_418647

Uniprot Accession Number

P0A7A9

Species

E.coli

AA Sequence

MSLLNVPAGK DLPEDIYVVI EIPANADPIK YEIDKESGAL FVDRFMSTAM FYPCNYGYIN HTLSLDGDPV DVLVPTPYPL QPGSVIRCRP VGVLKMTDEA GEDAKLVAVP HSKLSKEYDH IKDVNDLPEL LKAQIAHFFE HYKDLEKGKW VKVEGWENAE AAKAEIVASF ERAKNK