Recombinant human LC3B/MAP1LC3B protein
Microtubule-associated proteins 1A/1B light chain 3 beta 2, also as known as MAP1LC3B2, belongs to the MAP1LC3 family. MAP1LC3B2 is a subunit of neuronal microtubule-associated MAP1A and MAP1B proteins, which are involved in microtubule assembly and important for neurogenesis. This protein probably involved in formation of autophagosomal vacuoles (autophagosomes) . It is expressed primarily in heart, testis, brain and skeletal muscle and testis. Recombinant human MAP1LC3B2 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Specifications
Product Name Alternative
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
1 mg/mL (determined by Bradford assay)
Purity
> 95% by SDS-PAGE
Molecular Weight
16.2 kDa (140aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
He H., et al. (2003) J. Biol. Chem. 278:29278-29287; ; Seidenbecher C.I., et al. (2004) J. Mol. Biol. 336:957-970
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol, 0.1M NaCl
Scientific Category
Cytoskeleton
NCBI Accession Number
NP_001078950
Uniprot Accession Number
A6NCE7
Species
Human
AA Sequence
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