Recombinant human NDE1 protein

NDE1 is a cytoplasmic protein belonging to the nudE protein family. Phosphorylated during mitosis, it is essential for the formation and function of the mitotic spindle in M phase and functions to regulate the Dynein-mediated transport of kinetochore proteins, as well as centrosome duplication during interphase. NDE1 is thought to interact with NDEL1, LIS1 and Dynein IC1/2, cytosolic in a signaling pathway that regulates the formation of neurons and is fundamental to the development of the cerebral cortex. Recombinant human NDE1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Nuclear distribution protein nudE homolog 1, FLJ20101, HOM-TES-87, NuDE, NuDE, nudE nuclear distribution gene E homolog 1 (A. nidulans)

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.5 mg/mL (determined by Bradford assay)

Purity

> 85% by SDS-PAGE

Molecular Weight

39.9 kDa (355aa) confirmed by MALDI-TOF (Molecular weight on SDS-PAGE will appear higher)

Additionnal Information

NDE1, Nuclear distribution protein nudE homolog 1, FLJ20101, HOM-TES-87, NuDE, NuDE, nudE nuclear distribution gene E homolog 1 (A. nidulans), ATGP1027-10 µg, ATGP1027-20 µg, ATGP1027-50 µg, ATGP1027-100 µg, ATGP1027-250 µg, ATGP1027-500 µg, ATGP1027-1 mg, ATGP1027-10, ATGP1027-20, ATGP1027-50, ATGP1027-100, ATGP1027-250, ATGP1027-500, ATGP1027-1

References & Citations

Yan X., et al. (2003) Mol Cell Biol. 23 (4) :1239-50. ; ; Kitagawa M., et al. (2000) FEBS Lett. 479 (1-2) :57-62.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 20% glycerol, 5mM DTT, 0.2M NaCl.

Scientific Category

Cell Cycle

NCBI Accession Number

NP_001137451

Uniprot Accession Number

Q9NXR1

Species

Human

AA Sequence

MGSSHHHHHH SSGLVPRGSH MEDSGKTFSS EEEEANYWKD LAMTYKQRAE NTQEELREFQ EGSREYEAEL ETQLQQIETR NRDLLSENNR LRMELETIKE KFEVQHSEGY RQISALEDDL AQTKAIKDQL QKYIRELEQA NDDLERAKRA TIMSLEDFEQ RLNQAIERNA FLESELDEKE NLLESVQRLK DEARDLRQEL AVQQKQEKPR TPMPSSVEAE RTDTAVQATG SVPSTPIAHR GPSSSLNTPG SFRRGLDDST GGTPLTPAAR ISALNIVGDL LRKVGALESK LASCRNLVYD QSPNRTGGPA SGRSSKNRDG GERRPSSTSV PLGDKGLDTS CRWLSKSTTR SSSSC