Recombinant human GSKIP protein

GSKIP (GSK3 beta interaction protein) is defined as a naturally occurring protein that is homologous with the GSK3beta interaction domain of Axin and is able to negatively regulate GSK3beta of the Wnt signaling pathway. GSKIP may affect GSK3beta activity on neurite outgrowth by inhibiting the specific phosphorylation of tau (ser396) . Recombinant human GSKIP protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

GSK3-beta interaction protein

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 95% by SDS-PAGE

Molecular Weight

17.8 kDa (159aa) confirmed by MALDI-TOF

Additionnal Information

GSKIP, GSK3-beta interaction protein, ATGP1007-10 µg, ATGP1007-20 µg, ATGP1007-50 µg, ATGP1007-100 µg, ATGP1007-250 µg, ATGP1007-500 µg, ATGP1007-1 mg, ATGP1007-10, ATGP1007-20, ATGP1007-50, ATGP1007-100, ATGP1007-250, ATGP1007-500, ATGP1007-1

References & Citations

Lin CC, et al. (2009) J Cell Biochem. 108 (6) :1325-36.; ; Chou HY, et al (2006) Biochemistry. 45 (38) :11379-89.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol, 50 mM NaCl

Scientific Category

Metabolism

NCBI Accession Number

NP_057556

Uniprot Accession Number

Q9P0R6

Species

Human

AA Sequence

MGSSHHHHHH SSGLVPRGSH METDCNPMEL SSMSGFEEGS ELNGFEGTDM KDMRLEAEAV VNDVLFAVNN MFVSKSLRCA DDVAYINVET KERNRYCLEL TEAGLKVVGY AFDQVDDHLQ TPYHETVYSL LDTLSPAYRE AFGNALLQRL EALKRDGQS