Recombinant human eIF-1b/EIF1B protein
EIF1B is crucial for the scanning process in vitro. During the scanning process, EIF1B is a component of a complex involved in recognition of the initiator codon. Translation is also initiated by the role of EIF1B in regulating the activity of ribosomal subunits 43S, 48S and 40S. This protein enables 43S ribosomal complexes to discern between cognate and near-cognate initiation codons, sensing the nucleotide content of initiation codons. Recombinant human EIF1B protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Specifications
Product Name Alternative
Eukaryotic translation initiation factor 1B, GC20, Protein translation factor SUI1 homolog GC20
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
1 mg/mL (determined by Bradford assay)
Purity
> 95% by SDS-PAGE
Molecular Weight
15 kDa (133aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Pestova TV., et al. (2002) Genes Dev. 16 (22) :2906-22. ; ; Asano K., et al. (2000) Genes Dec. 14 (19) :2534-46.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.5) containing 10% glycerol, 2mM DTT, 0.1M NaCl
Scientific Category
Signal Transduction
NCBI Accession Number
NP_005866
Uniprot Accession Number
O60739
Species
Human
AA Sequence
MGSSHHHHHH SSGLVPRGSH MSTIQNLQSF DPFADATKGD DLLPAGTEDY IHIRIQQRNG RKTLTTVQGI ADDYDKKKLV KAFKKKFACN GTVIEHPEYG EVIQLQGDQR KNICQFLLEV GIVKEEQLKV HGF
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