Recombinant E.coli Ecotin protein

Ecotin (E. coli serine protease inhibitor) inhibits pancreatic serine proteases. This protein inhibits chymotrypsin, trypsin, elastases, factor X, kallikrein as well as a variety of other proteases. The strength of inhibition does not appear to be correlated with a particular protease specificity. Recombinant E. coli Ecotin protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Ecotin a serine protease inhibitor, ECK2201, eti, JW2197

Expression System

E.coli

Antigen Species

E.coli

Tag

His-Tag

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 95% by SDS-PAGE

Molecular Weight

18.3 kDa (163aa) confirmed by MALDI-TOF

Additionnal Information

Ecotin, Ecotin a serine protease inhibitor, ECK2201, eti, JW2197, ATGP0878-10 µg, ATGP0878-20 µg, ATGP0878-50 µg, ATGP0878-100 µg, ATGP0878-250 µg, ATGP0878-500 µg, ATGP0878-1 mg, ATGP0878-10, ATGP0878-20, ATGP0878-50, ATGP0878-100, ATGP0878-250, ATGP0878-500, ATGP0878-1

References & Citations

Lilla JN., et al. (2009) J Biol Chem. 284 (20) :13792-803.; ; Lund LR., et al. (2006) EMBO J. 25 (12) :2686-97.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 50 mM NaCl, 1mM DTT, 10% glycerol

Scientific Category

Other

NCBI Accession Number

NP_416713

Uniprot Accession Number

P23827

Species

E.coli

AA Sequence

MGSSHHHHHH SSGLVPRGSH MAESVQPLEK IAPYPQAEKG MKRQVIQLTP QEDESTLKVE LLIGQTLEVD CNLHRLGGKL ENKTLEGWGY DYYVFDKVSS PVSTMMACPD GKKEKKFVTA YLGDAGMLRY NSKLPIVVYT PDNVDVKYRV WKAEEKIDNA VVR