Recombinant human NIT2 protein

Omega-amidase NIT2, also known NIT2, belongs to the nitrilase superfamily. This protein has an omega-amidase activity. The role of omega-amidase is to remove potentially toxic intermediates by converting alpha-ketoglutaramate and alpha-ketosuccinamate to biologically useful alpha-ketoglutarate and oxaloacetate, respectively. Also Nit2, which is widely distributed in nature, has been suggested to be a tumor suppressor protein. Recombinant human NIT2 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Omega-amidase NIT2, Nitrilase homolog 2

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 95% by SDS-PAGE

Molecular Weight

33 kDa (299aa) confirmed by MALDI-TOF

Additionnal Information

NIT2, Omega-amidase NIT2, Nitrilase homolog 2, ATGP0819-10 µg, ATGP0819-20 µg, ATGP0819-50 µg, ATGP0819-100 µg, ATGP0819-250 µg, ATGP0819-500 µg, ATGP0819-1 mg, ATGP0819-10, ATGP0819-20, ATGP0819-50, ATGP0819-100, ATGP0819-250, ATGP0819-500, ATGP0819-1

References & Citations

Barqliw K T., et al. (2008) Biochemistry. 47 (51) :13514-23.; ; Camarqo A., et al. (2007) Plant Cell. 19 (11) :3491-503.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol, 0.1M NaCl

Scientific Category

Metabolism

NCBI Accession Number

NP_064587

Uniprot Accession Number

Q9NQR4

Species

Human

AA Sequence

MGSSHHHHHH SSGLVPRGSH MGSMTSFRLA LIQLQISSIK SDNVTRACSF IREAATQGAK IVSLPECFNS PYGAKYFPEY AEKIPGESTQ KLSEVAKECS IYLIGGSIPE EDAGKLYNTC AVFGPDGTLL AKYRKIHLFD IDVPGKITFQ ESKTLSPGDS FSTFDTPYCR VGLGICYDMR FAELAQIYAQ RGCQLLVYPG AFNLTTGPAH WELLQRSRAV DNQVYVATAS PARDDKASYV AWGHSTVVNP WGEVLAKAGT EEAIVYSDID LKKLAEIRQQ IPVFRQKRSD LYAVEMKKP