Recombinant human NIT2 protein
Omega-amidase NIT2, also known NIT2, belongs to the nitrilase superfamily. This protein has an omega-amidase activity. The role of omega-amidase is to remove potentially toxic intermediates by converting alpha-ketoglutaramate and alpha-ketosuccinamate to biologically useful alpha-ketoglutarate and oxaloacetate, respectively. Also Nit2, which is widely distributed in nature, has been suggested to be a tumor suppressor protein. Recombinant human NIT2 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Specifications
Product Name Alternative
Omega-amidase NIT2, Nitrilase homolog 2
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
1 mg/mL (determined by Bradford assay)
Purity
> 95% by SDS-PAGE
Molecular Weight
33 kDa (299aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Barqliw K T., et al. (2008) Biochemistry. 47 (51) :13514-23.; ; Camarqo A., et al. (2007) Plant Cell. 19 (11) :3491-503.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol, 0.1M NaCl
Scientific Category
Metabolism
NCBI Accession Number
NP_064587
Uniprot Accession Number
Q9NQR4
Species
Human
AA Sequence
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