Recombinant human 17 beta-HSD14/HSD17B14 protein
HSD17B14, also known as 17-beta-hydroxysteroid dehydrogenase 14, belongs to the 17-beta-HSD family of proteins, which regulate the availability of steroids within various tissues rough out the body. It exists as a homotetramer that localizes to the cytoplasm and is highly expressed in brain, placenta, liver and kidney. Recombinant human HSD17B14 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.
Product Specifications
Product Name Alternative
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
1 mg/mL (determined by Bradford assay)
Purity
> 95% by SDS-PAGE
Molecular Weight
32.4 kDa (306aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Grimwood J., et al. (2004), Nature., 428: 529-535.; ; Lukacik P., et al. (2007) . Biochem. J., 402: 419-427
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 20% glycerol, 0.1M NaCl, 1mM DTT
Scientific Category
Redox Proteins
NCBI Accession Number
NP_057330
Uniprot Accession Number
Q9BPX1
Species
Human
AA Sequence
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