Recombinant human ECH1 protein

ECH1 (enoyl coenzyme A hydratase 1) belongs to the hydratase/isomerase superfamily. This Protein shows high sequence similarity to enoyl-coenzyme A (CoA) hydratases of several species, particularly within a conserved domain characteristic of these proteins. It contains a C-terminal peroxisomal targeting sequence, localizes to both the peroxisome and the mitochondria. This enzyme involved in the auxiliary step of the fatty acid beta-oxidation pathway specifically functioning to catalyze the isomerization of 3-trans, 5-cis-dienoyl-CoA to 2-trans, 4-transdienoyl-CoA. Recombinant human ECH1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Enoyl Coenzyme A hydratase 1 peroxisomal, enoyl Coenzyme A hydratase 1 peroxisomal, HPXEL

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Molecular Weight

34.4 kDa (316aa) confirmed by MALDI-TOF

Additionnal Information

ECH1, enoyl Coenzyme A hydratase 1 peroxisomal, enoyl Coenzyme A hydratase 1 peroxisomal, HPXEL, ATGP0711-10 µg, ATGP0711-20 µg, ATGP0711-50 µg, ATGP0711-100 µg, ATGP0711-250 µg, ATGP0711-500 µg, ATGP0711-1 mg, ATGP0711-10, ATGP0711-20, ATGP0711-50, ATGP0711-100, ATGP0711-250, ATGP0711-500, ATGP0711-1

References & Citations

Goehler H., et al. (2004) Mol. Cell 15 (6) :853-65.; ; FitzPatrick DR., et al. (1995) Genomics 27 (3) :457-66.;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 1mM DTT, 50 mM NaCl

Scientific Category

Metabolism

NCBI Accession Number

AAH17408

Uniprot Accession Number

Q13011

Species

Human

AA Sequence

TGSSAQEAA SGVALGEAPD HSYESLRVTS AQKHVLHVQL NRPNKRNAMN KVFWREMVEC FNKISRDADC RAVVISGAGK MFTAGIDLMD MASDILQPKG DDVARISWYL RDIITRYQET FNVIERCPKP VIAAVHGGCI GGGVDLVTAC DIRYCAQDAF FQVKEVDVGL AADVGTLQRL PKVIGNQSLV NELAFTARKM MADEALGSGL VSRVFPDKEV MLDAALALAA EISSKSPVAV QSTKVNLLYS RDHSVAESLN YVASWNMSML QTQDLVKSVQ ATTENKELKT VTFSKL