Recombinant human eIF-4E/EIF4E protein

EIF4E, a member of eukaryotic initiation factor 4 families, modulates translation of maternal mRNAs in early embryos before the onset of zygotic transcription. This protein recognizes and binds to the 7 methyl GTP cap structure of eukaryotic mRNAs, thereby mediating the initiation of translation. It facilitates ribosome binding by inducing the unwinding of the mRNAs secondary structures. Recombinant human EIF4E protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Product Specifications

Product Name Alternative

Eukaryotic translation initiation factor 4E, CBP, EIF4E1, EIF4EL1, EIF4F, Eukaryotic translation initiation factor 4E, eIF-4F 25 kDa subunit, mRNA cap-binding protein

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.5 mg/mL (determined by Bradford assay)

Purity

> 85% by SDS-PAGE

Molecular Weight

27.2 kDa (237aa) confirmed by MALDI-TOF

Additionnal Information

EIF4E, Eukaryotic translation initiation factor 4E, CBP, EIF4E1, EIF4EL1, EIF4F, Eukaryotic translation initiation factor 4E, ATGP0663-10 µg, ATGP0663-20 µg, ATGP0663-50 µg, ATGP0663-100 µg, ATGP0663-250 µg, ATGP0663-500 µg, ATGP0663-1 mg, ATGP0663-10, ATGP0663-20, ATGP0663-50, ATGP0663-100, ATGP0663-250, ATGP0663-500, ATGP0663-1

References & Citations

Bendahmane A., et al. (2010) PLoS One. 5 (6) :e11313.; ; Pelletier J., et al. (2010) J Gen Virol. 91 (Pt 5) :1224-8.;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 1mM DTT

Scientific Category

Epigenomics (Transcription & Translation)

NCBI Accession Number

NP_001959.1

Uniprot Accession Number

P06730

Species

Human

AA Sequence

MATVEPETTP TPNPPTTEEE KTESNQEVAN PEHYIKHPLQ NRWALWFFKN DKSKTWQANL RLISKFDTVE DFWALYNHIQ LSSNLMPGCD YSLFKDGIEP MWEDEKNKRG GRWLITLNKQ QRRSDLDRFW LETLLCLIGE SFDDYSDDVC GAVVNVRAKG DKIAIWTTEC ENREAVTHIG RVYKERLGLP PKIVIGYQSH ADTATKSGST TKNRFVV