Recombinant human eIF-2 alpha/EIF2S1 protein

EIF2S1, also known as eIF2 alpha, is the alpha subunit of the translation initiation factor eIF2 complex which catalyzes the first regulated step of protein synthesis initiation, promoting the binding of the initiator tRNA to 40S ribosomal subunits. The phosphorylation state of eIF2S1 controls the rate of tRNA translation. When eIF2 alpha is not phosphorylated, translation occurs at a normal rate. However, upon phosphorylation by one of several kinases, eIF2S1 is stabilized, thus preventing the GDP/GTP exchange reaction and slowing translation. Recombinant human EIF2S1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Eukaryotic translation initiation factor 2 subunit alpha, EIF2, Eukaryotic translation initiation factor 2 subunit 1 alpha 35kDa, EIF-2alpha, EIF2A

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.5 mg/mL (determined by Bradford assay)

Purity

> 85% by SDS-PAGE

Molecular Weight

38.2 kDa (335aa) confirmed by MALDI-TOF

Additionnal Information

EIF2 a, Eukaryotic translation initiation factor 2 subunit 1, EIF-2, EIF-2A, EIF-2alpha, EIF2, EIF2A, Eukaryotic translation initiation factor 2 subunit 1 EIF 2 alpha, EIF 2, EIF 2A, EIF 2alpha, EIF2 alpha, Eukaryotic translation initiation factor 2 subunit 1 alpha 35kDa, Eukaryotic translation initiation factor 2 subunit 1 alpha, Eukaryotic translation initiation factor 2 subunit alpha., ATGP0581-10 µg, ATGP0581-20 µg, ATGP0581-50 µg, ATGP0581-100 µg, ATGP0581-250 µg, ATGP0581-500 µg, ATGP0581-1 mg, ATGP0581-10, ATGP0581-20, ATGP0581-50, ATGP0581-100, ATGP0581-250, ATGP0581-500, ATGP0581-1

References & Citations

Scheuner D, et al. (2005) Nat Med. 11 (7) :757-64; ; Boyce M. et al. (2005) Science. 307 (5711) :935-9;

Other References

Alvarez-Castelao B, et al. The switch-like expression of heme-regulated kinase 1 mediates neuronal proteostasis following proteasome inhibition. (Elife. 2020) {https://pubmed.ncbi.nlm.nih.gov/32329716/}

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol 0.1M NaCl

Scientific Category

Epigenomics (Transcription & Translation)

NCBI Accession Number

NP_004085.1

Uniprot Accession Number

P05198

Species

Human

AA Sequence

MPGLSCRFYQ HKFPEVEDVV MVNVRSIAEM GAYVSLLEYN NIEGMILLSE LSRRRIRSIN KLIRIGRNEC VVVIRVDKEK GYIDLSKRRV SPEEAIKCED KFTKSKTVYS ILRHVAEVLE YTKDEQLESL FQRTAWVFDD KYKRPGYGAY DAFKHAVSDP SILDSLDLNE DEREVLINNI NRRLTPQAVK IRADIEVACY GYEGIDAVKE ALRAGLNCST ENMPIKINLI APPRYVMTTT TLERTEGLSV LSQAMAVIKE KIEEKRGVFN VQMEPKVVTD TDETELARQM ERLERENAEV DGDDDAEEME AKAED