Recombinant human CDK2AP1 protein

CDK2AP1 is a specific CDK2-associated protein, which is thought to negatively regulate CDK2 activity by sequestering monomeric CDK2, and targeting CDK2 for proteolysis. Also, interact with DNA polymerase alpha/primase and mediate the phosphorylation of the large p180 subunit, which suggested the regulatory role in DNA replication during S phase of the cell cycle. A similar gene in hamster was isolated from, and functions as a growth suppressor of normal keratinocytes. Recombinant CDK2AP1 protein was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Cyclin-dependent kinase 2 associated protein 1, CDKAP1, DOC1, CDKA1, Cyclin-dependent kinase 2 associated protein 1 CDK2 A1, CDK2 associated protein 1, CDK2AP1, Cyclin dependent kinase 2 associated protein 1 Deleted in oral cancer 1, DOC 1, DOC 1 related protein, DOC 1R, DOC1R, Putative oral cancer suppressor.

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.25 mg/mL (determined by Bradford assay)

Purity

> 95% by SDS-PAGE

Molecular Weight

16.6 kDa (152aa) confirmed by MALDI-TOF

Additionnal Information

CDK2AP1, Cyclin-dependent kinase 2 associated protein 1, CDKAP1, DOC1, CDKA1, Cyclin-dependent kinase 2 associated protein 1 CDK2 A1, CDK2 associated protein 1, CDK2AP1, Cyclin dependent kinase 2 associated protein 1 Deleted in oral cancer 1, DOC 1, DOC 1 related protein, DOC 1R, DOC1R, Putative oral cancer suppressor., ATGP0480-10 µg, ATGP0480-20 µg, ATGP0480-50 µg, ATGP0480-100 µg, ATGP0480-250 µg, ATGP0480-500 µg, ATGP0480-1 mg, ATGP0480-10, ATGP0480-20, ATGP0480-50, ATGP0480-100, ATGP0480-250, ATGP0480-500, ATGP0480-1

References & Citations

Tsuji T., et al. (1998) J Biol Chem. 273 (12) :6704-9.; ; Hu MG., et al. (2004) Cancer Res. 64 (2) :490-9.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 50% glycerol, 2mM DTT

Scientific Category

Cell Cycle

NCBI Accession Number

NP_004633

Uniprot Accession Number

O14519

Species

Human

AA Sequence

MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSHMSY KPNLAAHMPA AALNAAGSVH SPSTSMATSS QYRQLLSDYG PPSLGYTQGT GNSQVPQSKY AELLAIIEEL GKEIRPTYAG SKSAMERLKR GIIHARGLVR ECLAETERNA RS