Recombinant human M-CSF protein
Macrophage colony stimulating factor (M-CSF), as known as CSF-1, one of the hematopoietic growth factors that regulate the growth and differentiation of blood cells. This protein is produced by monocytes, granulocytes, endothelial cells, and fibroblasts. It stimulates the formation of macrophage colonies, enhances antibody-dependent, cell-mediated cytotoxicity by monocytes and macrophages, and inhibits bone resorption by osteoclasts. Recombinant human M-CSF, fused to His-tag at N-terminus, was expressed as insoluble protein aggregate in E. coli and purified by conventional chromatography, after refolding of the isolated inclusion bodies in a renaturation buffer..
Product Specifications
Product Name Alternative
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
1 mg/mL (determined by Bradford assay)
Endotoxin
< 1 EU per 1ug of protein (determined by LAL method)
Purity
> 90% by SDS-PAGE
Molecular Weight
20.7 kDa (179aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Wei S., et al. (2006) . J Leukoc Biol. 80 (6) :1445-53.; ; Woo KM., et al. (2002) . Exp Mol Med. 34 (5) :340-6.;
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) 2mM DTT, 10% glycerol
Scientific Category
Hormones & Cytokines
NCBI Accession Number
NP_757351.2
Uniprot Accession Number
P09603
Species
Human
AA Sequence
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