Recombinant human VEGF 121/VEGFA protein
Vascular endothelial growth factor (VEGF) is homodimeric, heparin-binding glycoprotein involved in both angiogenesis and vasculogenesis. VEGF is expressed as multiple alternately spliced isoforms of VEGF121, 165, 189 and 206. VEGF binds to the receptor tyrosine kinases VEGF R1 (Flt-1) and VEGF R2 (KDR/Flk-1) to activate signal transduction and regulate both physiological and pathological angiogenesis. Recombinant VEGF121 protein was expressed in E. coli and purified by conventional chromatography, after refolding of the isolated inclusion bodies in a renaturation buffer.
Product Specifications
Specifications
Human VEGF121 stimulates cell proliferation of the HUVEC human umbilical vein endothelial cells. The ED50 range ≤4 ng/mL.
Product Name Alternative
Vascular endothelial growth factor A isoform f, Vascular permeability factor, VPF, VEGF
Expression System
E.coli
Antigen Species
Human
Tag
Non-Tagged
Applications
SDS-PAGE, Bioactivity
Concentration
0.5 mg/mL (determined by Bradford assay)
Endotoxin
< 1 EU per 1ug of protein (determined by LAL method)
Purity
> 95% by SDS-PAGE
Bioactivity
Measured in a cell proliferation assay using HUVEC human umbilical vein endothelial cells. The ED50 range ≤4 ng/mL.
Molecular Weight
14.2 kDa (122aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Yasuji ueda., et al. (2004) Cancer Chemother Pharmacol. 54 (1) :16-24.; ; Neufeld G., et al. (1999) FASEB J. 13 (1) :9-22.;
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10 % glycerol
Scientific Category
Hormones & Cytokines
NCBI Accession Number
NP_001020541.2
Uniprot Accession Number
P15692
Species
Human
AA Sequence
MAPMAEGGGQ NHHEVVKFMD VYQRSYCHPI ETLVDIFQEY PDEIEYIFKP SCVPLMRCGG CCNDEGLECV PTEESNITMQ IMRIKPHQGQ HIGEMSFLQH NKCECRPKKD RARQEKCDKP RR
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