Recombinant human VEGF 121/VEGFA protein

Vascular endothelial growth factor (VEGF) is homodimeric, heparin-binding glycoprotein involved in both angiogenesis and vasculogenesis. VEGF is expressed as multiple alternately spliced isoforms of VEGF121, 165, 189 and 206. VEGF binds to the receptor tyrosine kinases VEGF R1 (Flt-1) and VEGF R2 (KDR/Flk-1) to activate signal transduction and regulate both physiological and pathological angiogenesis. Recombinant VEGF121 protein was expressed in E. coli and purified by conventional chromatography, after refolding of the isolated inclusion bodies in a renaturation buffer.

Product Specifications

Specifications

Human VEGF121 stimulates cell proliferation of the HUVEC human umbilical vein endothelial cells. The ED50 range ≤4 ng/mL.

Product Name Alternative

Vascular endothelial growth factor A isoform f, Vascular permeability factor, VPF, VEGF

Expression System

E.coli

Antigen Species

Human

Tag

Non-Tagged

Applications

SDS-PAGE, Bioactivity

Concentration

0.5 mg/mL (determined by Bradford assay)

Endotoxin

< 1 EU per 1ug of protein (determined by LAL method)

Purity

> 95% by SDS-PAGE

Bioactivity

Measured in a cell proliferation assay using HUVEC human umbilical vein endothelial cells. The ED50 range ≤4 ng/mL.

Molecular Weight

14.2 kDa (122aa) confirmed by MALDI-TOF

Additionnal Information

VEGF121, ATGP0282-10 µg, ATGP0282-20 µg, ATGP0282-50 µg, ATGP0282-100 µg, ATGP0282-250 µg, ATGP0282-500 µg, ATGP0282-1 mg, ATGP0282-10, ATGP0282-20, ATGP0282-50, ATGP0282-100, ATGP0282-250, ATGP0282-500, ATGP0282-1