VHLL cDNA

Von Hippel-Lindau (VHL) tumor suppressor protein is a component of an E3 ubiquitin ligase complex that selectively ubiquitinates the alpha subunit of the hypoxia-inducible factor (HIF) transcription factor for proteasome-mediated degradation. Inactivation of VHL causes VHL disease and sporadic kidney cancer. This gene encodes a VHL homolog that lacks one of two key domains necessary for VHL function. This gene may contribute to the regulation of oxygen homeostasis and neovascularization during placenta development. This gene is intronless, and can also be interpreted as a retrotransposed pseudogene of the VHL locus located on chromosome 3. However, the protein is represented in this RefSeq due to evidence in PMID:14757845 that strongly suggests it is translated. The same publication also indicates that this protein binds HIF alpha but fails to recruit the E3 ubiquitin ligase complex, and it therefore functions as a dominant-negative VHL protein and a protector of HIF alpha.

Product Specifications

Product Name Alternative

VHLP, VLP

Chromosomal Location

1q22

Antigen Species

Human

Vector Type

PATGen (puc19-derived cloning vector)

Sequence

ATGCCCTGGAGAGCGGGGAACGGGGTGGGTTTAGAGGCCCAGGCGGGCACCCAGGAGGCAGGCCCAGAAGAGTACTGCCAGGAAGAGTTGGGCGCCGAGGAGGAGATGGCAGCCAGAGCAGCATGGCCTGTGCTGCGCTCTGTGAACTCACGCGAGCTCTCCCGGATCATCATCTGCAATCACAGCCCACGAATCGTGCTGCCTGTGTGGCTCAACTACTATGGCAAGCTGCTGCCCTACCTGACGCTGCTGCCCGGCAGGGACTTCCGCATCCACAACTTCCGAAGCCACCCTTGGCTCTTCAGAGATGCAAGGACACATGATAAGCTTCTGGTTAACCAAACTGAATTGTTTGTGCCATCTTCCAATGTTAATGGACAGCCTGTTTTTGCCAACATCACACTGCAGTGTATACCCTGA

Additionnal Information

VHLL, VHLP, VLP, VHLL, ATGD0187-10 µg, ATGD0187-20 µg, ATGD0187-50 µg, ATGD0187-100 µg, ATGD0187-250 µg, ATGD0187-500 µg, ATGD0187-1 mg, ATGD0187-10, ATGD0187-20, ATGD0187-50, ATGD0187-100, ATGD0187-250, ATGD0187-500, ATGD0187-1

Storage Conditions

Store the plasmid at -20C.

Formulation

Lyophilized

Scientific Category

Ubiquitination

NCBI Accession Number

NP_001004319.1

Species

Human

Preparation

1. Centrifuge at 7000rpm for 1 minute.; ;2. Carefully open the vial and add 100 µL of sterile water to dissolve the DNA.; ; Each tube contains approximately 10 µg of lyophilized plasmid.

RNA Reference

NM_001004319.1

DNA Size

420bp

Vector Description

This shuttle vector contains the complete ORF. It is inseted BamH I to Xho I. The gene insert contains multiple cloning sites which can be used to easily cut and transfer the gene and recombination site into your expression vector.