GALT cDNA

GALT catalyzes the second step of the Leloir pathway of galactose metabolism, namely the conversion of uDP-glucose + galactose-1-phosphate to glucose-1-phosphate + uDP-galactose. The absence of this enzyme results in classic galactosemia in humans and can be fatal in the newborn period if lactose is not removed from the diet. The pathophysiology of galactosemia has not been clearly defined. Two transcript variants encoding different isoforms have been found for this gene

Product Specifications

Chromosomal Location

9q13

Antigen Species

Human

Vector Type

PATGen (puc19-derived cloning vector)

Sequence

ATGTCGCGCA GTGGAACCGA TCCTCAGCAA CGCCAGCAGG CGTCAGAGGC GGACGCCGCA GCAGCAACCT TCCGGGCAAA CGACCATCAG CATATCCGCT ACAACCCGCT GCAGGATGAG TGGGTGCTGG TGTCAGCTCA CCGCATGAAG CGGCCCTGGC AGGGTCAAGT GGAGCCCCAG CTTCTGAAGA CAGTGCCCCG CCATGACCCT CTCAACCCTC TGTGTCCTGG GGCCATCCGA GCCAACGGAG AGGTGAATCC CCAGTACGAT AGCACCTTCC TGTTTGACAA CGACTTCCCA GCTCTGCAGC CTGATGCCCC CAGTCCAGGA CCCAGTGATC ATCCCCTTTT CCAAGCAAAG TCTGCTCGAG GAGTCTGTAA GGTCATGTGC TTCCACCCCT GGTCGGATGT AACGCTGCCA CTCATGTCGG TCCCTGAGAT CCGGGCTGTT GTTGATGCAT GGGCCTCAGT CACAGAGGAG CTGGGTGCCC AGTACCCTTG GGTGCAGATC TTTGAAAACA AAGGTGCCAT GATGGGCTGT TCTAACCCCC ACCCCCACTG CCAGGTATGG GCCAGCAGTT TCCTGCCAGA TATTGCCCAG CGTGAGGAGC GATCTCAGCA GGCCTATAAG AGTCAGCATG GAGAGCCCCT GCTAATGGAG TACAGCCGCC AGGAGCTACT CAGGAAGGAA CGTCTGGTCC TAACCAGTGA GCACTGGTTA GTACTGGTCC CCTTCTGGGC AACATGGCCC TACCAGACAC TGCTGCTGCC CCGTCGGCAT GTGCGGCGGC TACCTGAGCT GACCCCTGCT GAGCGTGATG ATCTAGCCTC CATCATGAAG AAGCTCTTGA CCAAGTATGA CAACCTCTTT GAGACGTCCT TTCCCTACTC CATGGGCTGG CATGGGGCTC CCACAGGATC AGAGGCTGGG GCCAACTGGA ACCATTGGCA GCTGCACGCT CATTACTACC CTCCGCTCCT GCGCTCTGCC ACTGTCCGGA AATTCATGGT TGGCTACGAA ATGCTTGCTC AGGCTCAGAG GGACCTCACC CCTGAGCAGG CTGCAGAGAG ACTAAGGGCA CTTCCTGAGG TTCATTACCA CCTGGGGCAG AAGGACAGGG AGACAGCAAC CATCGCCTGA

Additionnal Information

GALT, GALT, ATGD0007-10 µg, ATGD0007-20 µg, ATGD0007-50 µg, ATGD0007-100 µg, ATGD0007-250 µg, ATGD0007-500 µg, ATGD0007-1 mg, ATGD0007-10, ATGD0007-20, ATGD0007-50, ATGD0007-100, ATGD0007-250, ATGD0007-500, ATGD0007-1

Storage Conditions

Store the plasmid at -20C.

Formulation

Lyophilized

Scientific Category

Metabolism

Omim

606999

NCBI Accession Number

NP_000146.2

Species

Human

Preparation

1. Centrifuge at 7000rpm for 1 minute.; ;2. Carefully open the vial and add 100 µL of sterile water to dissolve the DNA.; ; Each tube contains approximately 10 µg of lyophilized plasmid.

RNA Reference

NM_000155.3

DNA Size

1140bp

Vector Description

This shuttle vector contains the complete ORF. It is inseted BamH I to Hind III. The gene insert contains multiple cloning sites which can be used to easily cut and transfer the gene and recombination site into your expression vector.