Human PDCD4 antibody

Programmed cell death 4 (PDCD4) encodes a tumor suppressor protein whose expression is lost in progressed carcinomas of lung, breast, colon, and prostate. The expression of PDCD4 gene is strongly induced during apoptosis in a number of cell types. It is modulated by cytokines in natural killer and T cells.

Product Specifications

Product Name Alternative

Programmed cell death 4 isoform 1, neoplastic transformation inhibitor, H731, MGC33046, MGC33047, Programmed cell death 4 isoform 1, PDPC4, Programmed cell death 4 isoform 1, PDCD4, programmed cell death 4 (neoplastic transformation inhibitor), nuclear antigen H731

Host

Mouse

Antigen Species

Human

Reactivity

Human

Immunogen

Recombinant human PDCD4 (1-469aa) purified from E. coli

Clonality

Monoclonal

Isotype

IgG1 κ

Clone

4C2

Conjugation

Unconjugated

Applications

ELISA, WB, ICC/IF, FACS

Purification Method

By protein-A affinity chromatography

Concentration

1 mg/mL (determined by BCA assay)

Additionnal Information

ATGA0601-10 µg, ATGA0601-20 µg, ATGA0601-50 µg, ATGA0601-100 µg, ATGA0601-250 µg, ATGA0601-500 µg, ATGA0601-1 mg, ATGA0601-10, ATGA0601-20, ATGA0601-50, ATGA0601-100, ATGA0601-250, ATGA0601-500, ATGA0601-1

References & Citations

Palamarchuk A, et al., (2006) Cancer Res 65 (24) :11282-11286.; ; Yoshina, et al., (1999) Pathol Int 49 (12) :1067-1077.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Applications Notes

The antibody has been tested by ELISA, Western blot, ICC/IF and FACS analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Scientific Category

Cancer, Epigenetics and Nuclear Signaling

NCBI Accession Number

NP_055271

Uniprot Accession Number

Q53EL6

WB Description

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human PDCD4 antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: K562 cell lysate; ; Lane 2.: HeLa cell lysate; ; Lane 3.: 293T cell lysate

FACS Description

Flow cytometry analysis of PDCD4 in MCF7 cells. The cell was stained with ATGA0601 at 2-5ug for 1x10^6cells (red) . A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray) .

IF Description

ICC/IF analysis of PDCD4 in HeLa cells. The cell was stained with ATGA0601 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .