GAPDH antibody

Multiple roles for glyceraldehyde-3-phosphate dehydrogenase (GAPDH) have been recently appreciated. GAPDH is found in the particulate fractions, such as the nucleus, the mitochondria, and the small vesicular fractions. GAPDH gene expression is specifically increased during programmed neuronal cell death. When cells are exposed to various stressors, dynamic subcellular re-distribution of GAPDH occurs. GAPDH is also involved in various diseases, especially neurodegenerative disorders and cancers. As a membrane protein, GAPDH functions in endocytosis in the cytoplasm, it is involved in the translational control of gene expression in the nucleus, it functions in nuclear tRNA export, in DNA replication, and in DNA repair.

Product Specifications

Product Name Alternative

Glyceraldehyde-3-phosphate dehydrogenase isoform 1, Peptidyl-cysteine S-nitrosylase GAPDH, GAPD, G3PD

Host

Mouse

Antigen Species

Human

Immunogen

GAPDH from rabbit muscle

Clonality

Monoclonal

Isotype

IgG2b κ

Clone

AT4E8

Conjugation

Unconjugated

Applications

ELISA, WB, ICC/IF

Purification Method

By protein-A affinity chromatography

Concentration

0.2 mg/mL (determined by BCA assay)

Additionnal Information

ATGA0394, wu:fb33a10, Peptidyl-cysteine S-nitrosylase GAPDH, Peptidyl cysteine S nitrosylase GAPDH, p38 component, OTTHuMP00000174432, OTTHuMP00000174431, OCT1 coactivator in S phase, OCAS, MGC88685, MGC127711, MGC105239, MGC103191, MGC103190, MGC102546, MGC102544, KNC-NDS6, KNC NDS6, HEL-S-162eP, HEL S 162eP, Glyceraldehyde-3-phosphate dehydrogenase isoform 2, Glyceraldehyde-3-phosphate dehydrogenase isoform 1, Glyceraldehyde-3-phosphate dehydrogenase 38 kDa BFA-dependent ADP-ribosylation substrate, Glyceraldehyde-3-phosphate dehydrogenase, Glyceraldehyde3 phosphate dehydrogenase, Glyceraldehyde 3 phosphate dehydrogenase isoform 2, Glyceraldehyde 3 phosphate dehydrogenase isoform 1, Glyceraldehyde 3 phosphate dehydrogenase 38 kDa BFA dependent ADP ribosylation substrate, Glyceraldehyde 3 phosphate dehydrogenase, GAPDH human, GAPDH, GAPD, G3PDH, G3PD, EC1.2.1.12, EC 1.2.1.12, cb609, BARS-38, BARS 38, Aging-associated gene 9 protein, Aging associated gene 9 protein, 38-KD component, 38

References & Citations

Chuang DM., et al, (2005) Annu Rev Pharmacol Toxicol. 45:269-90.; ; Mazzola JL., et al, (2002) Neurotoxicology. 23 (4-5) :603-9.; ; Sirover MA. (1997) J Cell Biochem. 66 (2) :133-40.

Other References

Chang Y, et al. Clinical impact of serum exosomal microRNA in liver fibrosis. (PLoS One. 2021) {https://pubmed.ncbi.nlm.nih.gov/34506494/}; ; Hah YS, et al. β-Sitosterol Attenuates Dexamethasone-Induced Muscle Atrophy via Regulating FoxO1-Dependent Signaling in C2C12 Cell and Mice Model. (Nutrients. 2022) {https://pubmed.ncbi.nlm.nih.gov/35889851/}; ; Hah YS, et al. Rutin Prevents Dexamethasone-Induced Muscle Loss in C2C12 Myotube and Mouse Model by Controlling FOXO3-Dependent Signaling. (Antioxidants (Basel) . 2023) {https://pubmed.ncbi.nlm.nih.gov/36978887/}; ; Park SW, et al. Development of new tools to study membrane-anchored mammalian Atg8 proteins. (Autophagy. 2023) {https://pubmed.ncbi.nlm.nih.gov/36250672/}

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Applications Notes

The antibody has been tested by ELISA, Western blot and ICC/IF analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Scientific Category

Neuroscience

NCBI Accession Number

NP_001075722.1

Uniprot Accession Number

P46406

WB Description

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-GAPDH antibody (1:200) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: HepG2 cell lysate; ; Lane 2.: MCF7 cell lysate; ; Lane 3.: Jurkat cell lysate; ; Lane 4.: A549 cell lysate The tissue lysates (20 µg) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-GAPDH antibody (1:200) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: Mouse Brain tissue lysate; ; Lane 2.: Mouse Eye tissue lysate; ; Lane 3.: Mouse Muscle tissue lysate{2008240001438589.jpg}The 293T cell lysate (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human GAPDH. Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: Anti-GAPDH monoclonal antibody (1:20,000) ; ; Lane 2.: Anti-GAPDH monoclonal antibody (1:10,000) ; ; Lane 3.: Anti-GAPDH monoclonal antibody (1:2,000) ; ; Lane 4.: Anti-GAPDH monoclonal antibody (1:200) {2008240001620935.jpg}

IF Description

ICC/IF analysis of GAPDH in HeLa cells. The cell was stained with ATGA0592 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .