Human STEAP1 antibody

Six-transmembrane epithelial antigen of the prostate (STEAP) is expressed predominantly in human prostatetissue and in other common malignancies including prostate, bladder, colon, and ovarian carcinomas, and in Ewing's sarcoma, suggesting that it could function as an almost universal tumor antigen. Immunohistochemical analysisindicated STEAP-1 localization at cell-cell junctions of the secretory epithelium of prostate and prostatecancer cells. The favorable expression profiling of STEAP-1 in normal and cancer tissues suggested itspotential use as a target for immunotherapy.

Product Specifications

Product Name Alternative

Six transmembrane epithelial antigen of the prostate 1, six transmembrane epithelial antigen of the prostate, PRSS24

Host

Mouse

Antigen Species

Human

Reactivity

Human

Immunogen

Recombinant human STEAP1 (1-70aa) purified from E. coli

Clonality

Monoclonal

Isotype

IgG2b κ

Clone

J2D2

Conjugation

Unconjugated

Applications

ELISA, WB

Purification Method

By protein-A affinity chromatography

Concentration

1 mg/mL (determined by BCA assay)

Additionnal Information

STEAP1, ATGA0551-10 µg, ATGA0551-20 µg, ATGA0551-50 µg, ATGA0551-100 µg, ATGA0551-250 µg, ATGA0551-500 µg, ATGA0551-1 mg, ATGA0551-10, ATGA0551-20, ATGA0551-50, ATGA0551-100, ATGA0551-250, ATGA0551-500, ATGA0551-1

References & Citations

David A. Rodeberg, et al. (2005) Clin Cancer Res. June11 (12) : 4545-4552.; ; Pia M. Challita-Eid, et al., (2007) Cancer research June 67 (12) : 5798-5805.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Applications Notes

The antibody has been tested by ELISA and Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Scientific Category

Epigenetics and Nuclear Signaling

NCBI Accession Number

NP_036581

Uniprot Accession Number

Q9UHE8

WB Description

The cell lysate (40ug) was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human STEAP1 antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: U87MG cell lysate;