Human CD34 antibody

Hematopoietic progenitor cell antigen CD34 is a heavily glycosylated, transmembrane glycoprotein that is expressed on the surface of lymphohematopoietic stem and progenitor cells, small-vessel endothelial cells, embryonic fibroblasts and some cells in fetal and adult nervous tissue. It is likely an adhesion molecule with a role in early hematopoiesis by mediating attachment of stem cells to the bone marrow extracellular matrix or directly to stromal cells.

Product Specifications

Product Name Alternative

Hematopoietic progenitor cell antigen CD34, CD34 molecule

Host

Mouse

Antigen Species

Human

Reactivity

Human

Immunogen

Recombinant human CD34 (32-290aa) purified from E.coli

Clonality

Monoclonal

Isotype

IgG2a κ

Clone

AT64F9

Conjugation

Unconjugated

Applications

ELISA, WB, FACS

Purification Method

By protein-A affinity chromatography

Concentration

1 mg/mL (determined by BCA assay)

Additionnal Information

CD34, ATGA0500-10 µg, ATGA0500-20 µg, ATGA0500-50 µg, ATGA0500-100 µg, ATGA0500-250 µg, ATGA0500-500 µg, ATGA0500-1 mg, ATGA0500-10, ATGA0500-20, ATGA0500-50, ATGA0500-100, ATGA0500-250, ATGA0500-500, ATGA0500-1

References & Citations

Satterthwaite A B., et al. (1992) Genomics. 12:788-794.; ; Krause D S., et al. (1994) Blood. 84:691-701.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Applications Notes

The antibody has been tested by ELISA, Western blot and FACS analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Scientific Category

Neuroscience

NCBI Accession Number

NP_001020280

Uniprot Accession Number

P28906

WB Description

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human CD34 antibody (1:500) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: TF1 cell lysate; ; Lane 2.: HeLa cell lysate; ; Lane 3.: Jurkat cell lysate

FACS Description

Flow cytometry analysis of CD34 in KG1 cells. The cell was stained with ATGA0500 at 2-5ug for 1x10^6cells (red) . A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray) .