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Human TOMM20 antibody

Mitochondrial import receptor subunit TOMM20 homolog, also known as TOMM20, belongs to the Tom20 family. The Tom machinery consists of import receptors for the initial binding of cytosolically synthesized preproteins and a general import pore (GIP) for the membrane translocation of various preproteins into the mitochondria. TOMM20 functions as the transit peptide receptor at the surface of the mitochondrion outer membrane and facilitates the movement of preproteins into the TOM40 translocation pore.

Product Specifications

Product Name Alternative

Translocase of outer mitochondrial membrane 20, Translocase of outer mitochondrial membrane 20 homolog, Translocase of outer mitochondrial membrane 20 homolog type II, Mitochondrial import receptor subunit TOM20 homolog, Mitochondrial 20 kDa outer membrane protein, Outer mitochondrial membrane receptor Tom20, TOM20, MOM19, MAS20

Host

Mouse

Antigen Species

Human

Reactivity

Human

Immunogen

Recombinant human Tomm20 (25-145aa) purified from E. coli

Clonality

Monoclonal

Isotype

IgG1 κ

Clone

AT1B2

Conjugation

Unconjugated

Applications

ELISA, WB, ICC/IF, FACS

Purification Method

By protein-A affinity chromatography

Concentration

1 mg/mL (determined by BCA assay)

Additionnal Information

Tomm20, ATGA0482-10 µg, ATGA0482-20 µg, ATGA0482-50 µg, ATGA0482-100 µg, ATGA0482-250 µg, ATGA0482-500 µg, ATGA0482-1 mg, ATGA0482-10, ATGA0482-20, ATGA0482-50, ATGA0482-100, ATGA0482-250, ATGA0482-500, ATGA0482-1

References & Citations

Ahting U., et al. (1999) J Cell Biol. 147: 959-968.; ; Brix J., et al. (1999) J Biol Chem. 274: 16522-16530.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Applications Notes

The antibody has been tested by ELISA, Western blot, ICC/IF and FACS analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Scientific Category

Signal Transduction

NCBI Accession Number

NP_055580

Uniprot Accession Number

Q15388

WB Description

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Tomm20 antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: HeLa cell lysate; ; Lane 2.: HepG2 cell lysate; ; Lane 3.: A431 cell lysate; ; Lane 4.: K562 cell lysate; ; Lane 5.: A549 cell lysate; ; Lane 6.: 293T cell lysate; ; Lane 7.: MCF7 cell lysate; ; Lane 8.: SK-OV-3 cell lysate; ; Lane 9.: PC3 cell lysate;

FACS Description

Flow cytometry analysis of Tomm20 in HeLa cells. The cell was stained with ATGA0482 at 2-5ug for 1x10^6cells (red) . A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray) .

IF Description

ICC/IF analysis of Tomm20 in HeLa cells. The cell was stained with ATGA0482 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .
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