Human HSPA13 antibody

HSPA13, also known as STCH, is a member of the heat shock protein 70 family and is found associated with microsomes. Members of this protein family play a role in the processing of cytosolic and secretory proteins, as well as in the removal of denatured or incorrectly-folded proteins. HSPA13 has been found to interact with PLIC-1 and PLIC-2, proteins involved in the signaling connection between the membrane receptors for thrombospondin and the cytoskeleton.

Product Specifications

Product Name Alternative

Heat shock protein 70kDa family member 13, STCH

Host

Mouse

Antigen Species

Human

Reactivity

Human

Immunogen

Recombinant human HSPA13 (23-471aa) purified from E. coli

Clonality

Monoclonal

Isotype

IgG1 κ

Clone

AT2F6

Conjugation

Unconjugated

Applications

ELISA, WB, ICC/IF

Purification Method

By protein-A affinity chromatography

Concentration

1 mg/mL (determined by BCA assay)

Additionnal Information

HSPA13, Heat shock protein 70kDa family member 13, STCH, ATGA0477-10 µg, ATGA0477-20 µg, ATGA0477-50 µg, ATGA0477-100 µg, ATGA0477-250 µg, ATGA0477-500 µg, ATGA0477-1 mg, ATGA0477-10, ATGA0477-20, ATGA0477-50, ATGA0477-100, ATGA0477-250, ATGA0477-500, ATGA0477-1

References & Citations

Rual JF., et al. (2005) Nature. 437 (7062) : 1173-8.; ; Lim J., et al. (2006) Cell. 125 (4) : 801-14.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Applications Notes

The antibody has been tested by ELISA, Western blot and ICC/IF analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Scientific Category

Signal Transduction

NCBI Accession Number

AAH36370

Uniprot Accession Number

P48723

WB Description

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human HSPA13 antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ; ; Lane 1.: 293T cell lysate ; ; Lane 2.: K562 cell lysate ; ; Lane 3.: MCF7 cell lysate

IF Description

ICC/IF analysis of HSPA13 in Hep3B cells. The cell was stained with ATGA0477 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .