Welcome to GenPrice! Check out our latest updates.

Shopping Cart (0)

Your cart is empty

Add some products to get started!

Human PSMD11 antibody

The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. PSMD11 is a member of the proteasome subunit S9 family that functions as a nonATPase subunit of the 19S regulator and is phosphorylated by AMP-activated protein kinase.

Product Specifications

Product Name Alternative

Proteasome 26S non-ATPase subunit 11, S9, Rpn6, p44.5, MGC3844

Host

Mouse

Antigen Species

Human

Reactivity

Human

Immunogen

Recombinant human PSMD11 (1-422aa) purified from E. coli

Clonality

Monoclonal

Isotype

IgG2b κ

Clone

AT2C7

Conjugation

Unconjugated

Applications

ELISA, WB, ICC/IF

Purification Method

By protein-A affinity chromatography

Concentration

1 mg/mL (determined by BCA assay)

Additionnal Information

PSMD11, ATGA0446-10 µg, ATGA0446-20 µg, ATGA0446-50 µg, ATGA0446-100 µg, ATGA0446-250 µg, ATGA0446-500 µg, ATGA0446-1 mg, ATGA0446-10, ATGA0446-20, ATGA0446-50, ATGA0446-100, ATGA0446-250, ATGA0446-500, ATGA0446-1

References & Citations

Ogris E., et al. (1999) J. Biol. Chem. 274: 14382-14391.; ; Gagnon S.N., et al. (2002) Biochem. J. 368: 263-271.

Other References

Fukuyama K, et al. Roasted Coffee Reduces β-Amyloid Production by Increasing Proteasomal β-Secretase Degradation in Human Neuroblastoma SH-SY5Y Cells. (Mol Nutr Food Res. 2018) {https://pubmed.ncbi.nlm.nih.gov/30144352/}

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Applications Notes

The antibody has been tested by ELISA, Western blot analysis and ICC/IF to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Scientific Category

Ubiquitination

NCBI Accession Number

NP_002806

Uniprot Accession Number

O00231

WB Description

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human PSMD11 antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: HeLa cell lysate; ; Lane 2.: 293T cell lysate; ; Lane 3.: U87-MG cell lysate; ; Lane 4.: NIH-3T3 cell lysate; ; Lane 5.: PC3 cell lysate; ; Lane 6.: TF1 cell lysate; ; Lane 7.: MCF7 cell lysate

IF Description

ICC/IF analysis of PSMD11 in HeLa cells. The cell was stained with ATGA0446 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .
Curated Selection

Explore Other Products

Discover premium biology products from our extensive collection of 20M+ items

Mouse SESN3 (Sestrin 3) ELISA Kit
EM23861 96 Tests

Mouse SESN3 (Sestrin 3) ELISA Kit

Ask
View Details
Bovine Tumor Necrosis Factor Alpha (TNF-alpha) ELISA Kit
MBS2609886-01 48 Well

Bovine Tumor Necrosis Factor Alpha (TNF-alpha) ELISA Kit

Ask
View Details
Bovine Tumor Necrosis Factor Alpha (TNF-alpha) ELISA Kit
MBS2609886-02 96 Well

Bovine Tumor Necrosis Factor Alpha (TNF-alpha) ELISA Kit

Ask
View Details
Bovine Tumor Necrosis Factor Alpha (TNF-alpha) ELISA Kit
MBS2609886-03 5x 96 Well

Bovine Tumor Necrosis Factor Alpha (TNF-alpha) ELISA Kit

Ask
View Details
Bovine Tumor Necrosis Factor Alpha (TNF-alpha) ELISA Kit
MBS2609886-04 10x 96 Well

Bovine Tumor Necrosis Factor Alpha (TNF-alpha) ELISA Kit

Ask
View Details
Human JAM-B/VE-JAM Alexa Fluor 700 MAb (Clone 988901)
FAB10742N-100UG 100 µg

Human JAM-B/VE-JAM Alexa Fluor 700 MAb (Clone 988901)

Ask
View Details