Human ACAA1 antibody

ACAA1 is a member of the thiolase family of enzymes and is involved in lipid metabolism. This protein is localized to the peroxisome and catalyzes the conversion of acyl-CoA and acetyl-CoA to 3-oxoacyl-CoA in the fatty acid oxidation pathway. ACAA1 shows high enzymatic activity in liver, kidney, intestine and white adipose tissue in rats. Deficiency of this enzyme leads to pseudo-Zellweger syndrome.

Product Specifications

Product Name Alternative

3-ketoacyl-CoA thiolase peroxisomal, ACAA, PTHIO, THIO, 3-ketoacyl-CoA thiolase, peroxisomal

Host

Mouse

Antigen Species

Human

Reactivity

Human

Immunogen

Recombinant human ACAA1 (27-424aa) purified from E. coli

Clonality

Monoclonal

Isotype

IgG2b κ

Clone

AT9E5

Conjugation

Unconjugated

Applications

ELISA, WB, ICC/IF

Purification Method

By protein-A affinity chromatography

Concentration

1 mg/mL (determined by BCA assay)

Additionnal Information

ACAA1, 3-ketoacyl-CoA thiolase peroxisomal, ACAA, PTHIO, THIO, 3-ketoacyl-CoA thiolase, peroxisomal, ATGA0429-10 µg, ATGA0429-20 µg, ATGA0429-50 µg, ATGA0429-100 µg, ATGA0429-250 µg, ATGA0429-500 µg, ATGA0429-1 mg, ATGA0429-10, ATGA0429-20, ATGA0429-50, ATGA0429-100, ATGA0429-250, ATGA0429-500, ATGA0429-1

References & Citations

Bour A., et al. (1991) Biochim Biophys Acta. 1090 (1) : 43-51.; ; Patel S., et al. (2003) Eur Respir J. 22 (5) : 755-60.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Applications Notes

The antibody has been tested by ELISA, Western blot analysis, Flow cytometry and ICC/IF to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Scientific Category

Signal Transduction

NCBI Accession Number

NP_001598

Uniprot Accession Number

P09110

WB Description

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human ACAA1 antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ; ; Lane 1 : HepG2 cell lysate ; ; Lane 2 : LnCap cell lysate

IF Description

ICC/IF analysis of ACAA1 in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human ACAA1 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green) .