Human GOT2 antibody

Glutamic-oxaloacetic transaminase is a pyridoxal phosphate-dependent enzyme which exists in cytoplasmic and inner-membrane mitochondrial forms, GOT1 and GOT2, respectively. GOT plays a role in amino acid metabolism and the urea and tricarboxylic acid cycles. Also, GOT2 is a major participant in the malate-aspartate shuttle, which is a passage from the cytosol to the mitochondria. The two enzymes are homodimeric and show close homology. GOT2 has been seen to have a role in cell proliferation, especially in terms of tumor growth.

Product Specifications

Product Name Alternative

Aspartate aminotransferase 2 mitochondrial, Aspartate aminotransferase 2, mitochondrial, KAT4, KATIV, mitAAT

Host

Mouse

Antigen Species

Human

Reactivity

Human

Immunogen

Recombinant human GOT2 (30-430aa) purified from E.coli

Clonality

Monoclonal

Isotype

IgG3 κ

Clone

AT8B11

Conjugation

Unconjugated

Applications

ELISA, WB, ICC/IF, FACS

Purification Method

By protein-A affinity chromatography

Concentration

1 mg/mL (determined by BCA assay)

Additionnal Information

GOT2, ATGA0357-10 µg, ATGA0357-20 µg, ATGA0357-50 µg, ATGA0357-100 µg, ATGA0357-250 µg, ATGA0357-500 µg, ATGA0357-1 mg, ATGA0357-10, ATGA0357-20, ATGA0357-50, ATGA0357-100, ATGA0357-250, ATGA0357-500, ATGA0357-1

References & Citations

Yang H., et al. (2015) The EMBO journal. 34 (8) : 1110-1125.; ; Guidetti P., et al. (2007) Journal of neurochemistry. 102 (1) : 103-111.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Applications Notes

The antibody has been tested by ELISA, Western blot, ICC/IF and FACS analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Scientific Category

Metabolism

NCBI Accession Number

NP_002071

Uniprot Accession Number

P00505

WB Description

The recombinant human GOT2 (50ng), cell and tissue lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human GOT2 antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: GOT2 Recombinant protein; ; Lane 2.: Ramos cell lysate; ; Lane 3.: 293T cell lysate; ; Lane 4.: Liver tissue lysate The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human GOT2 antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: HeLa cell lysate; ; Lane 2.: HepG2 cell lysate; ; Lane 3.: Balb/3T3 cell lysate {ATGA0357-WB2.jpg} The recombinant proteins (20ng) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human GOT2 antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: Recombinant Human GOT1; ; Lane 2.: Recombinant Human GOT2 {ATGA0357-WB3.jpg}

FACS Description

Flow cytometry analysis of GOT2 in HepG2 cell line, staining at 2-5ug for 1x10^6cells (red line) . The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line) .

IF Description

ICC/IF analysis of GOT2 in HeLa cells. The cell was stained with ATGA0357 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .