Human CD46 antibody

Membrane Cofactor Protein (CD46) is a type 1 membrane protein that plays an important inhibitory role in the complement system. CD46 regulates the complement cascade by inhibiting C3b and C4b deposited on self tissue. This protein is a cofactor that binds to C3b and C4b, allowing their degradation by a plasma serine protease called factor I. This function resides in the complement control protein repeats (CCPs), with CCP1-4 essential for regulation. CD46 is implicated in the development and/or progression of selected cancer types.

Product Specifications

Product Name Alternative

Membrane cofactor protein isoform 14, AHuS2, MCP, MIC10, TLX, TRA2.10

Host

Mouse

Antigen Species

Human

Reactivity

Human

Immunogen

Recombinant human CD46 (35-313aa) purified from E. coli

Clonality

Monoclonal

Isotype

IgG2a κ

Clone

AT2G9

Conjugation

Unconjugated

Applications

ELISA, WB, ICC/IF, IHC, FACS

Purification Method

By protein-A affinity chromatography

Concentration

1 mg/mL (determined by BCA assay)

Additionnal Information

CD46, ATGA0326-10 µg, ATGA0326-20 µg, ATGA0326-50 µg, ATGA0326-100 µg, ATGA0326-250 µg, ATGA0326-500 µg, ATGA0326-1 mg, ATGA0326-10, ATGA0326-20, ATGA0326-50, ATGA0326-100, ATGA0326-250, ATGA0326-500, ATGA0326-1

References & Citations

Liszewski M.K., et al. (1991) Annu Rev Immunol 9: 431-455.; ; Riley-Varqas R.C., et al. (2004) Trends Immunol 25 (9) : 496-503.; ; Studebaker A.W., et al. (2010) Neuro Oncol 12: 1034-1042.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Applications Notes

The antibody has been tested by ELISA, Western blot, ICC/IF, FACS and IHC analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Scientific Category

Immunology

NCBI Accession Number

NP_758860

Uniprot Accession Number

P15529

WB Description

The cell lysates of Ramos, HeLa and A549 (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human CD46 antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

IHC description

Paraffin embedded sections of human colorectal cancer tissue were incubated with anti-human CD46 (1:100) for 2 hours at room temperature. Antigen retrieval was performed in 0.1M sodium citrate buffer and detected using Diaminobenzidine (DAB)

FACS Description

Flow cytometry analysis of CD46 in PBMC cells. The cell was stained with ATGA0326 at 2-5ug for 1x10^6cells (red) . A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray) .

IF Description

ICC/IF analysis of CD46 in HeLa cells. The cell was stained with ATGA0326 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .