Human TBCB antibody

Tubulin folding cofactor B, also known as TBCB, belongs to the Microtubules family. Biosynthesis of functional microtubules involve the participation of several chaperones, termed Tubulin folding cofactors A (TBCA), B (TBCB), D (TBCD), E (TBCE) and C (TBCC), that act on folding intermediates downstream of the cytosolic chaperon, alternatively named TCP. TBCB is a 244 amino acid cytoplasmic protein containing one CAP-Gly domain and in widely expressed. TBCB is involved in the regulation of tubulin heterodimer dissociation and may function as a negative regulator of axonal growth.

Product Specifications

Product Name Alternative

Tubulin folding cofactor B, CG22, CKAP1, CKAPI

Host

Mouse

Antigen Species

Human

Reactivity

Human

Immunogen

Recombinant human TBCB (1-244aa) purified from E. coli

Clonality

Monoclonal

Isotype

IgG2b κ

Clone

AT1F6

Conjugation

Unconjugated

Applications

ELISA, WB, FACS, ICC/IF

Purification Method

By protein-A affinity chromatography

Concentration

1 mg/mL (determined by BCA assay)

Additionnal Information

TBCB, Tubulin folding cofactor B, CG22, CKAP1, CKAPI, ATGA0302-10 µg, ATGA0302-20 µg, ATGA0302-50 µg, ATGA0302-100 µg, ATGA0302-250 µg, ATGA0302-500 µg, ATGA0302-1 mg, ATGA0302-10, ATGA0302-20, ATGA0302-50, ATGA0302-100, ATGA0302-250, ATGA0302-500, ATGA0302-1

References & Citations

Kortazar D., et al. (2007) Exp Cell Res. 313:425-436.; ; Tian G., et al. (1997) J Cell Biol. 138: 821-832; ; Fanarraga ML., et al. (2009) Exp cell Res 315:535-541;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Applications Notes

The antibody has been tested by ELISA, Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended starting dilution is 1:1500

Scientific Category

Cytoskeleton

NCBI Accession Number

NP_001272

Uniprot Accession Number

Q99426

WB Description

The cell lysates of Jurkat (40ug) and A549 (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human TBCB antibody (1:1500) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

FACS Description

Flow cytometry analysis of TBCB in HeLa cells. The cell was stained with ATGA0302 at 2-5ug for 1x106cells (red) . A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray) .

IF Description

ICC/IF analysis of TBCB in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human TBCB antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green) .