Human eIF-2 alpha/EIF2S1 antibody

Eukaryotic translation initiation factor 2, subunit 1 alpha (EIF2S1) is plays a central role in initiating translation, and provides for a rate-limiting step in protein synthesis. The phosphorylation of alpha-subunit of EIF2S1 effectively prevents formation of the EIF2S1/GTP/Met-tRNAi complex and inhibits global protein synthesis.

Product Specifications

Product Name Alternative

Eukaryotic translation initiation factor 2 subunit alpha, EIF2, Eukaryotic translation initiation factor 2 subunit 1 alpha 35kDa, EIF-2alpha, EIF2A

Host

Mouse

Antigen Species

Human

Reactivity

Human

Immunogen

Recombinant human EIF2S1 (1-315aa) purified from E. coli

Clonality

Monoclonal

Isotype

IgG2b κ

Clone

AT5E10

Conjugation

Unconjugated

Applications

ELISA, WB, ICC/IF

Purification Method

By protein-G affinity chromatography

Concentration

1 mg/mL (determined by BCA assay)

Additionnal Information

EIF2S1, ATGA0208-10 µg, ATGA0208-20 µg, ATGA0208-50 µg, ATGA0208-100 µg, ATGA0208-250 µg, ATGA0208-500 µg, ATGA0208-1 mg, ATGA0208-10, ATGA0208-20, ATGA0208-50, ATGA0208-100, ATGA0208-250, ATGA0208-500, ATGA0208-1

References & Citations

Kim JH, et al. (2011) . EMBO J, 1030 (12) :2454-64.; ; Pena J, et al. (2011) . J Biol Chem, 286 (16) :14226-36.; ; Muaddi H, et al. (2010) . Mol Biol Cell, 21 (18) :3220-31.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Applications Notes

The antibody has been tested by ELISA, Western blot and ICC/IF analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Scientific Category

Signal Transduction

NCBI Accession Number

NP_004085

Uniprot Accession Number

P05198

WB Description

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human EIF2S1 antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: HeLa cell lysate ; ; Lane 2.: Jurkat cell lysate ; ; Lane 3.: NIH-3T3 cell lysate

IF Description

ICC/IF analysis of EIF2S1 in HeLa cells. The cell was stained with ATGA0208 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .