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Human ARF1 antibody

ADP-ribosylation factor 1 (ARF1) is a member of the human ARF gene family. The family member is essential and ubiquitous in eukaryotes, being involved in vesicular transport and functioning as an activator of phospholipase D and cholera toxin. The functions of ARF proteins in membrane traffic and organelle integrity are intimately tied to its reversible association with membranes and specific interactions with membrane phospholipids. ARF1 is various membrane trafficking events in the ER-Golgi system and in the maintenance of organelle structure. Inactive ARF1 (ARF1-GDP) localizes in the cytoplasm, while the active form (ARF1-GTP) localizes in the membrane.

Product Specifications

Product Name Alternative

ADP-ribosylation factor 1, ADP ribosylation factor 1

Host

Mouse

Antigen Species

Human

Reactivity

Human

Immunogen

Recombinant human ARF1 (1-181aa) purified from E. coli

Clonality

Monoclonal

Isotype

IgG1 κ

Clone

AT1B3

Conjugation

Unconjugated

Applications

ELISA, WB, ICC/IF, FACS

Purification Method

By protein-G affinity chromatography

Concentration

1 mg/mL (determined by BCA assay)

Additionnal Information

ARF1, ATGA0207-10 µg, ATGA0207-20 µg, ATGA0207-50 µg, ATGA0207-100 µg, ATGA0207-250 µg, ATGA0207-500 µg, ATGA0207-1 mg, ATGA0207-10, ATGA0207-20, ATGA0207-50, ATGA0207-100, ATGA0207-250, ATGA0207-500, ATGA0207-1

References & Citations

Amor, J. C. et al. (1994) Nature 372: 704-708.; ; Claude, A. et al. (1999) J. Cell Biol. 146: 71-84.; ; Derrien, V. et al. (2002) J. Cell Sci. 115: 2867-2879.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Applications Notes

The antibody has been tested by ELISA, Western blot, ICC/IF and FACS analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Scientific Category

Signal Transduction

NCBI Accession Number

NP_001649

Uniprot Accession Number

P84077

WB Description

The cell lysates and tissue lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human ARF1 antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: HeLa cell lysate; ; Lane 2.: HepG2 cell lysate; ; Lane 3.: Brain tissue lysate; ; Lane 4.: MCF7 cell lysate

FACS Description

Flow cytometry analysis of ARF1 in MCF7 cell line, staining at 2-5ug for 1x10^6cells (red line) . The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line) .

IF Description

ICC/IF analysis of ARF1 in MCF7 cells. The cell was stained with ATGA0207 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .ICC/IF analysis of ARF1 in U87MG cells. The cell was stained with ATGA0207 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .{ATGA0207-IF2.jpg}
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