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Human BAK antibody

BAK1 encodes an receptor-like kinase (RLK) with a putative extracellular domain, a single transmembrane domain, an intracellular-juxtamembrane domain, and a kinase domain. BAK1 expression is associated with the progression of Prostate cancer (CaP) . BAK1 appears to function in distinct receptor-signaling complexes to integrate multiple microbe-associated molecular pattern (MAMP) perception into downstream-signaling events. BAK1 can serve multiple purposes to simultaneously modulate MAMP-receptor complexes, BR signaling, and cell death for the benefit of bacterial infection, life style, and fitness.

Product Specifications

Product Name Alternative

BCL2-antagonist/killer 1, Apoptosis regulator BAK, BAK, BAK-LIKE, Bcl-2 homologous antagonist/killer, Bcl2-L-7, BAK-1, BAK 1, BAK like, Bak NT, BAK1, Bcl 2 homologous antagonist/killer, Bcl 2 like 7 protein, Bcl2 homologous antagonist killer, Bcl2 like 7 Protein, BCL2-antagonist/killer 1, BCL2L7, CDN 1, CDN1, Cell death inhibitor 1, MGC117255, MGC3887, NBak, Pro apoptotic protein BAK

Host

Mouse

Antigen Species

Human

Reactivity

Human

Immunogen

Recombinant human BAK1 (29-187aa) purified from E.coli

Clonality

Monoclonal

Isotype

IgG2a κ

Clone

AT38E2

Conjugation

Unconjugated

Applications

ELISA, WB, ICC/IF, FACS

Purification Method

By protein-G affinity chromatography

Concentration

1 mg/mL (determined by BCA assay)

Additionnal Information

BAK, ATGA0183-10 µg, ATGA0183-20 µg, ATGA0183-50 µg, ATGA0183-100 µg, ATGA0183-250 µg, ATGA0183-500 µg, ATGA0183-1 mg, ATGA0183-10, ATGA0183-20, ATGA0183-50, ATGA0183-100, ATGA0183-250, ATGA0183-500, ATGA0183-1

References & Citations

Birgit Kemmerling, et al. (2008) Plant Signal Behav, 3 (2) : 116-118.; ; Libo Shan, et al. (2008) Cell Host Microbe, 4 (1) : 17-27.; ; Xu-Bao Shi, et al. (2007) Proc Natl Acad Sci U S A, 104 (50) : 19983-19988.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Applications Notes

The antibody has been tested by ELISA, Western blot, ICC/IF and FACS analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Scientific Category

Apoptosis

NCBI Accession Number

NP_001179

Uniprot Accession Number

Q16611

WB Description

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human BAK1 antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1 : 293T cell lysate; ; Lane 2 : HeLa cell lysate; ; Lane 3 : A431 cell lysate; ; Lane 4 : A549 cell lysate; ; Lane 5 : Jurkat cell lysate; ; Lane 6 : MCF7 cell lysate; ; Lane 7 : PC3 cell lysate

FACS Description

Flow cytometry analysis of BAK1 in HeLa cell line, staining at 2-5ug for 1x10^6cells (red line) . The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line) .

IF Description

ICC/IF analysis of BAK1 in HeLa cells. The cell was stained with ATGA0183 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .
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