Human GAPDH antibody

Multiple roles for glyceraldehyde-3-phosphate dehydrogenase (GAPDH) have been recently appreciated. GAPDH is found in the particulate fractions, such as the nucleus, the mitochondria, and the small vesicular fractions. GAPDH gene expression is specifically increased during programmed neuronal cell death. When cells are exposed to various stressors, dynamic subcellular re-distribution of GAPDH occurs. GAPDH is also involved in various diseases, especially neurodegenerative disorders and cancers. As a membrane protein, GAPDH functions in endocytosis; in the cytoplasm, it is involved in the translational control of gene expression; in the nucleus, it functions in nuclear tRNA export, in DNA replication, and in DNA repair.

Product Specifications

Product Name Alternative

Glyceraldehyde-3-phosphate dehydrogenase isoform 1, Peptidyl-cysteine S-nitrosylase GAPDH, GAPD, G3PD

Host

Mouse

Antigen Species

Human

Reactivity

Human

Immunogen

Recombinant human GAPDH (1-335aa) purified from E.coli

Clonality

Monoclonal

Isotype

IgG2b κ

Clone

AT8G4

Conjugation

Unconjugated

Applications

ELISA, WB, ICC/IF

Purification Method

By protein-G affinity chromatography

Concentration

1 mg/mL (determined by BCA assay)

Additionnal Information

Wu:fb33a10, Peptidyl-cysteine S-nitrosylase GAPDH, Peptidyl cysteine S nitrosylase GAPDH, p38 component, OTTHuMP00000174432, OTTHuMP00000174431, OCT1 coactivator in S phase, OCAS, MGC88685, MGC127711, MGC105239, MGC103191, MGC103190, MGC102546, MGC102544, KNC-NDS6, KNC NDS6, HEL-S-162eP, HEL S 162eP, Glyceraldehyde-3-phosphate dehydrogenase isoform 2, Glyceraldehyde-3-phosphate dehydrogenase isoform 1, Glyceraldehyde-3-phosphate dehydrogenase 38 kDa BFA-dependent ADP-ribosylation substrate, Glyceraldehyde-3-phosphate dehydrogenase, Glyceraldehyde3 phosphate dehydrogenase, Glyceraldehyde 3 phosphate dehydrogenase isoform 2, Glyceraldehyde 3 phosphate dehydrogenase isoform 1, Glyceraldehyde 3 phosphate dehydrogenase 38 kDa BFA dependent ADP ribosylation substrate, Glyceraldehyde 3 phosphate dehydrogenase, GAPDH human, GAPDH, GAPD, G3PDH, G3PD, EC1.2.1.12, EC 1.2.1.12, cb609, BARS-38, BARS 38, Aging-associated gene 9 protein, Aging associated gene 9 protein, 38-KD component, 38 KD compone

References & Citations

Chuang DM, et al. (2005) Annu Rev Pharmacol Toxicol, 45:269-90.; ; Mazzola JL, et al. (2002) Neurotoxicology, 23 (4-5) :603-9.; ; Sirover MA. (1997) J Cell Biochem, 66 (2) :133-40.

Other References

Migas AA, et al. RUNX1T1/MTG8/ETO gene expression status in human t (8;21) (q22; q22) -positive acute myeloid leukemia cells. (Leuk Res. 2014) {https://pubmed.ncbi.nlm.nih.gov/24976338/}; ; Yu H, et al. Sulfasalazine‑induced ferroptosis in breast cancer cells is reduced by the inhibitory effect of estrogen receptor on the transferrin receptor. (Oncol Rep. 2019) {https://pubmed.ncbi.nlm.nih.gov/31173262/}

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Applications Notes

The antibody has been tested by ELISA, Western blot and ICC/IF analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Scientific Category

Cancer

NCBI Accession Number

NP_002037

Uniprot Accession Number

P04406

WB Description

The HeLa cell lysates (35ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human GAPDH antibody. Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: Anti-human GAPDH antibody (1:1000) ; ; Lane 2.: Anti-human GAPDH antibody (1:2000) ; ; Lane 3.: Anti-human GAPDH antibody (1:4000) ; ; Lane 4.: Anti-human GAPDH antibody (1:6000) ; ; Lane 5.: Anti-human GAPDH antibody (1:8000) ; ; Lane 6.: Anti-human GAPDH antibody (1:10000) ;

IF Description

Immunofluorescence of human HeLa cells stained with Hoechst 3342 (Blue) for nucleus staining and monoclonal anti-human GAPDH antibody (1:500) with Texas Red