Human OAT antibody

OAT is a 49-kDa nucleus-encoded protein imported into mitochondria to give the mature 48-kDa OAT polypeptide. It has been described in humans, animals, insects, plants and microorganisms. Especially OAT have sex-differential expression in the mouse kidney. OAT plays crucial physiological roles in amino acid metabolism. OAT shows a large structural and mechanistic similarity to other enzymes from the subgroup III of aminotransferases, which transfer an amino group from a carbon atom that does not carry a carboxyl function. OAT is essential for nitrogen recycling from arginine but not for the stress-induced proline accumulation.

Product Specifications

Product Name Alternative

Ornithine aminotransferase precursor, DKFZp781A11155, HOGA, OATASE, ornithine aminotransferase precursor EC 2.6.1.13, ornithine aminotransferase (gyrate atrophy), ornithine aminotransferase precursor, Ornithine aminotransferase, mitochondrial precursor, Ornithine oxo-acid aminotransferase, Ornithine--oxo-acid aminotransferase

Host

Mouse

Antigen Species

Human

Reactivity

Human

Immunogen

Recombinant human OAT (33-439aa) purified from E.coli

Clonality

Monoclonal

Isotype

IgG1 κ

Clone

AT23A2

Conjugation

Unconjugated

Applications

ELISA, WB, ICC/IF

Purification Method

By protein-G affinity chromatography

Concentration

1 mg/mL (determined by BCA assay)

Additionnal Information

OAT, ATGA0176-10 µg, ATGA0176-20 µg, ATGA0176-50 µg, ATGA0176-100 µg, ATGA0176-250 µg, ATGA0176-500 µg, ATGA0176-1 mg, ATGA0176-10, ATGA0176-20, ATGA0176-50, ATGA0176-100, ATGA0176-250, ATGA0176-500, ATGA0176-1

References & Citations

Stranska J, et al. (2008) Plant Signal Behav, 3 (11) :929-35.; ; Canas RA, et al. (2008) Plant Physiol, 148 (1) :77-88.; ; Levillain O, et al. (2007) Am J Physiol Renal Physiol, 292 (3) :F1016-27.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Applications Notes

The antibody has been tested by ELISA, Western blot and ICC/IF analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Scientific Category

Metabolism

NCBI Accession Number

NP_000265

Uniprot Accession Number

P04181

WB Description

Tissue lysate of mouse liver (35ug) was resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human OAT (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. The recombinant protein (20ng) and lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human OAT antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: OAT recombinant protein; ; Lane 2.: 293T cell lysate; ; Lane 3.: Mouse brain tissue lysate {ATGA0176-WB2.jpg}

IF Description

ICC/IF analysis of OAT in HeLa cells. The cell was stained with ATGA0176 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .ICC/IF analysis of OAT in Hep3B cells. The cell was stained with ATGA0176 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .{ATGA0176-IF2.jpg}