Human MAT2A antibody

Methionine adenosyltransferase II (MAT II) is a key enzyme in cellular metabolism and catalyzes the formation of S-adenosylmethionine (SAMe) from L-methionine and ATP. MAT2A is expressed in extrahepatic tissues. In liver, MAT2A expression associates with growth, dedifferentiation, and cancer.

Product Specifications

Product Name Alternative

Methionine adenosyltransferase II alpha, MATA2, MATII, SAMS2, Methionine adenosyltransferase II alpha, MAT2A, Methionine adenosyltransferase II, alpha AdoMet synthetase, AdoMet synthetase 2, AMS 2, MAT II, MATA 2, Methionine adenosyltransferase, Methionine adenosyltransferase 2, SAMS 2, Methionine adenosyltransferase II, S adenosylmethionine synthetase gamma form, S adenosylmethionine synthetase isoform type 2, S-adenosylmethionine synthetase isoform type 2

Host

Mouse

Antigen Species

Human

Reactivity

Human

Immunogen

Recombinant human MAT2A (1-395aa) purified from E. coli

Clonality

Monoclonal

Isotype

IgG2b κ

Clone

AT3A2

Conjugation

Unconjugated

Applications

ELISA, WB, ICC/IF, IHC

Purification Method

By protein-G affinity chromatography

Concentration

1 mg/mL (determined by BCA assay)

Additionnal Information

MAT2A, ATGA0138-10 µg, ATGA0138-20 µg, ATGA0138-50 µg, ATGA0138-100 µg, ATGA0138-250 µg, ATGA0138-500 µg, ATGA0138-1 mg, ATGA0138-10, ATGA0138-20, ATGA0138-50, ATGA0138-100, ATGA0138-250, ATGA0138-500, ATGA0138-1

References & Citations

Ramani K, et al., (2008) Hepatology 47 (2) :521-531.; ; Chen H, et al., (2007) Gastroenterology 133 (1) :207-218.

Other References

Ikeda S, et al, Kume S. Role of methionine adenosyltransferase 2A in bovine preimplantation development and its associated genomic regions. (Sci Rep. 2017) {https://pubmed.ncbi.nlm.nih.gov/28630431/}

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Applications Notes

The antibody has been tested by ELISA, Western blot, ICC/IF and IHC analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Scientific Category

Metabolism

NCBI Accession Number

NP_005902

Uniprot Accession Number

P31153

WB Description

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human MAT2A antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: HeLa cell lysate; ; Lane 2.: Ramos cell lysate; ; Lane 3.: 293T cell lysate; ; Lane 4.: Jurkat cell lysate; ; Lane 5.: NIH-3T3 cell lysate;

IHC description

Paraffin embedded sections of human colon cancer tissue were incubated with anti-human MAT2A (1:50) for 2 hours at room temperature. Antigen retrieval was performed in 0.1M sodium citrate buffer and detected using Diaminobenzidine (DAB)

IF Description

ICC/IF analysis of MAT2A in HeLa cells. The cell was stained with ATGA0138 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .