Human eIF-5A1/EIF5A antibody

Eukaryotic translation initiation factor 5A (eIF5A) is the only protein Known to contain unusual amino acid formed by the action of deoxyhypusine synthase and deoxyhypusine hydroxylase using spermidine as the substrate. This protein was previously reported to be involved in the first step of peptide bond formation in translation; however more recent work implicates it as a universally conserved translation elongation factor. Modulation of eIF5A has been linked to proliferation and cancer.

Product Specifications

Product Name Alternative

Eukaryotic translation initiation factor 5A-1 isoform B, Eukaryotic translation initiation factor 5A, Eukaryotic translation initiation factor 5A-1, eIF-5A-1, Eukaryotic initiation factor 5A isoform 1, eIF-5A, Rev-binding factor, eIF-4D

Host

Mouse

Antigen Species

Human

Reactivity

Human

Immunogen

Recombinant human EIF5A (1-154aa) purified from E. coli

Clonality

Monoclonal

Isotype

IgG1 κ

Clone

AT4D1

Conjugation

Unconjugated

Applications

ELISA, WB, ICC/IF, FACS

Purification Method

By protein-G affinity chromatography

Concentration

1 mg/mL (determined by BCA assay)

Additionnal Information

EIF5A, ATGA0128-10 µg, ATGA0128-20 µg, ATGA0128-50 µg, ATGA0128-100 µg, ATGA0128-250 µg, ATGA0128-500 µg, ATGA0128-1 mg, ATGA0128-10, ATGA0128-20, ATGA0128-50, ATGA0128-100, ATGA0128-250, ATGA0128-500, ATGA0128-1

References & Citations

Saini P., et al. (2009) Nature. 459 (7243) : 118-21.; ; Gosslau A., et al. (2009) J Cell Physiol. 219 (2) : 485-93.;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Applications Notes

The antibody has been tested by ELISA, Western blot, ICC/IF and FACS analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Scientific Category

Epigenomics (Transcription & Translation)

NCBI Accession Number

NP_001961

Uniprot Accession Number

P63241

WB Description

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human EIF5A antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: Jurkat cell lysate; ; Lane 2.: 293T cell lysate; ; Lane 3.: HeLa cell lysate; ; Lane 4.: MCF7 cell lysate; ; Lane 5.: NIH-3T3 cell lysate

FACS Description

Flow cytometry analysis of EIF5A in Balb/3T3 cell line, staining at 2-5ug for 1x10^6cells (red line) . The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line) .

IF Description

ICC/IF analysis of EIF5A in Balb/3T3 cells. The cell was stained with ATGA0128 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .