Human STEAP1 antibody

Six-transmembrane epithelial antigen of the prostate (STEAP) is expressed predominantly in human prostate tissue and in other common malignancies including prostate, bladder, colon, and ovarian carcinomas, and in Ewings sarcoma, suggesting that it could function as an almost universal tumor antigen. Immunohistochemical analysis indicated STEAP-1 localization at cell-cell junctions of the secretory epithelium of prostate and prostate cancer cells. The favorable expression profiling of STEAP-1 in normal and cancer tissues suggested its potential use as a target for immunotherapy.

Product Specifications

Product Name Alternative

Six transmembrane epithelial antigen of the prostate 1, six transmembrane epithelial antigen of the prostate, PRSS24

Host

Mouse

Antigen Species

Human

Reactivity

Human

Immunogen

Recombinant human STEAP1 (1-70aa) purified from E. coli

Clonality

Monoclonal

Isotype

IgG2b κ

Clone

J2D2

Conjugation

Unconjugated

Applications

ELISA, WB, ICC/IF, IHC

Purification Method

By protein-G affinity chromatography

Concentration

1 mg/mL (determined by BCA assay)

Additionnal Information

STEAP1, AST0910-10 µg, AST0910-20 µg, AST0910-50 µg, AST0910-100 µg, AST0910-250 µg, AST0910-500 µg, AST0910-1 mg, AST0910-10, AST0910-20, AST0910-50, AST0910-100, AST0910-250, AST0910-500, AST0910-1

References & Citations

David A. Rodeberg, et al. (2005) Clin Cancer Res. June 11 (12) : 4545-4552.; ; Pia M. Challita-Eid, et al., (2007) Cancer research June 67 (12) : 5798-5805.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Applications Notes

The antibody has been tested by ELISA, Western blot, ICC/IF and IHC analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Scientific Category

Cancer

NCBI Accession Number

NP_036581

Uniprot Accession Number

Q9UHE8

WB Description

The cell lysates (30ug) and tissue lysates (30ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human STEAP1 antibody (1:500) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: LNcap cell lysate; ; Lane 2.: Mouse Testis Tissue lysate;

IHC description

Paraffin embedded sections of human breast cancer and prostate hyperplasia tissue were incubated with anti-human STEAP1 (1:50) for 2 hours at room temperature. Antigen retrieval was performed in 0.1M sodium citrate buffer and detected using Diaminobenzidine (DAB) {AST0910-IHC1b.jpg}

IF Description

ICC/IF analysis of STEAP1 in LNCap cells. The cell was stained with AST0910 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .