Human SETD7 antibody

SET 7/9 is a histone methyltransferase (HMTase) that transfers methyl group to Lys4 of histone H3, in complex with S-adenosyl-L-methionine (AdoMet) . The methylation of lysine residues of histones plays a critical role in the regulation of chromatin structure and gene expression. Acetylation, phosphorylation and methylation of the amino-terminal tails of histone are thought to be involved in the regulation of chromatin structure and function. The enzymes identified in the methylation of specific lysine residue on histones belong to the SET family with just one exception. Set7/9, unlike most other SET proteins, is exclusively a mono-methylase.

Product Specifications

Product Name Alternative

SETD7, SET7, SET9, SET7/9, SET7/9 Histone methyltransferase, SET domain-containing protein 8, SET domain-containing protein 7 FLJ21193, SET domain-containing protein 7, Lysine N-methyltransferase 7, Lysine methyltransferase, KMT7, KIAA1717, Histone-lysine N-methyltransferase SETD7, Histone-lysine N-methyltransferase, Histone lysine N methyltransferase H3 lysine 4 specific SET7, Histone lysine methyltransferase, Histone H4-K4 methyltransferase, Histone H3-K4 methyltransferase SETD7, Histone H3 lysine 4 specific methyltransferase, Histone H3 K4 methyltransferase, H4 lysine-4 specific, H3-K4-HMTase SETD7, H3 K4 HMTase, EC 2.1.1.43

Host

Mouse

Antigen Species

Human

Reactivity

Human

Immunogen

Recombinant human SET 7/9 (1-366aa) purified from E. coli

Clonality

Monoclonal

Isotype

IgG2b κ

Clone

S4E5

Conjugation

Unconjugated

Applications

ELISA, WB, ICC/IF, FACS

Purification Method

By protein-A affinity chromatography

Concentration

1 mg/mL (determined by BCA assay)

Additionnal Information

SETD7, SET9, SET7/9 Histone methyltransferase, SET7/9, SET7, SET domain-containing protein 8, SET domain-containing protein 7 FLJ21193, SET domain-containing protein 7, SET domain containing protein 7, SET D7, SET 9, SET 7/9, SET 7, Lysine N-methyltransferase 7, Lysine methyltransferase, KMT7, KIAA1717, Histone-lysine N-methyltransferase SETD7, Histone-lysine N-methyltransferase, Histone lysine N methyltransferase H3 lysine 4 specific SET7, Histone lysine methyltransferase, Histone H4-K4 methyltransferase, Histone H3-K4 methyltransferase SETD7, Histone H3 lysine 4 specific methyltransferase, Histone H3 K4 methyltransferase, H4 lysine-4 specific, H3-K4-HMTase SETD7, H3 K4 HMTase, EC 2.1.1.43, AHR0401-10 µg, AHR0401-20 µg, AHR0401-50 µg, AHR0401-100 µg, AHR0401-250 µg, AHR0401-500 µg, AHR0401-1 mg, AHR0401-010, AHR0401-020, AHR0401-050, AHR0401-100, AHR0401-250, AHR0401-500, AHR0401-01M

References & Citations

Bing X, et al., (2003) Nature 421: 652-56.; ; Taewoo K, et al., (2003) EMBO. 22: 292-303.; ; Nishioka K, et al., (2002) Genes Dev. 16: 479-89.

Other References

Kurash JK, et al. Methylation of p53 by Set7/9 mediates p53 acetylation and activity in vivo. (Mol Cell. 2008) {https://pubmed.ncbi.nlm.nih.gov/18280244/}; ; Tao Y, et al. The histone methyltransferase Set7/9 promotes myoblast differentiation and myofibril assembly. (J Cell Biol. 2011) {https://pubmed.ncbi.nlm.nih.gov/21859860/}; ; Han T, et al. Set7 facilitates hepatitis C virus replication via enzymatic activity-dependent attenuation of the IFN-related pathway. (J Immunol. 2015) {https://pubmed.ncbi.nlm.nih.gov/25681344/}; ; Zhang Y, et al. The transcription factor GATA1 and the histone methyltransferase SET7 interact to promote VEGF-mediated angiogenesis and tumor growth and predict clinical outcome of breast cancer. (Oncotarget. 2016) {https://pubmed.ncbi.nlm.nih.gov/26848522/}; ; Yuan H, et al. Epigenetic Histone Modifications Involved in Profibrotic Gene Regulation by 12/15-Lipoxygenase and Its Oxidized Lipid Products in Diabetic Nephropathy. (Antioxid Redox Signal. 2016) {https://pubmed.ncbi.nlm.nih.gov/26492974/}; ; Xu X, et al. A signature motif in LIM proteins mediates binding to checkpoint proteins and increases tumour radiosensitivity. (Nat Commun. 2017) {https://pubmed.ncbi.nlm.nih.gov/28094252/}

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Applications Notes

The antibody has been tested by ELISA, Western blot, ICC/IF and FACS analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtainoptimal results.

Scientific Category

Signal Transduction

NCBI Accession Number

NP_085151

Uniprot Accession Number

Q8WTS6

WB Description

The recombinant human SET 7/9 protein was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human SET 7/9 antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. The cell lysates (5ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human SET 7/9 antibody (1:2000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: 293T cell lysate; ; Lane 2.: SET 7/9 transfected 293T cell lysate {AHR0401-WB2.jpg}

FACS Description

Flow cytometry analysis of SET 7/9 in Jurkat cell line, staining at 2-5ug for 1x10^6cells (red line) . The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line) .

IF Description

ICC/IF analysis of SET 7/9 in HeLa cells. The cell was stained with AHR0401 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .