Human Dapper 3/DACT3 antibody

DACT3, a member of the DACT (Dpr/Frodo) gene family, plays an important role in a negative regulator of Wnt/-catenin signaling. Unlike other Wnt signaling inhibitors that are silenced by DNA methylation, DACT3 repression is associated with bivalent histone modifications.

Product Specifications

Product Name Alternative

Thymus expressed gene 3-like, Dapper antagonist of beta-catenin homolog 3 (Xenopus laevis), Arginine rich region 1, MGC15476

Host

Mouse

Antigen Species

Human

Reactivity

Human

Immunogen

Recombinant human DACT3 (89-202aa) purified from E. coli

Clonality

Monoclonal

Isotype

IgG1 κ

Clone

2A5

Conjugation

Unconjugated

Applications

ELISA, WB, IHC

Purification Method

By protein-G affinity chromatography

Concentration

1 mg/mL (determined by BCA assay)

Additionnal Information

DACT3, ADA0925-10 µg, ADA0925-20 µg, ADA0925-50 µg, ADA0925-100 µg, ADA0925-250 µg, ADA0925-500 µg, ADA0925-1 mg, ADA0925-10, ADA0925-20, ADA0925-50, ADA0925-100, ADA0925-250, ADA0925-500, ADA0925-1

References & Citations

Jiang X, et al., (2008) Cancer Cell. 13 (6) :529-41.; ; Fisher DA, et al., (2006) Dev Dyn. 235 (9) :2620-30.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Applications Notes

The antibody has been tested by ELISA, Western blot and IHC analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Scientific Category

Development

NCBI Accession Number

NP_659493

Uniprot Accession Number

Q96B18

WB Description

The tissue lysate (40ug) was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human DACT3 antibody (1:250) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: Mouse Brain tissue lysate

IHC description

Paraffin embedded sections of human gastric cancer tissue were incubated with anti-human DACT3 (1:50) for 2 hours at room temperature. Antigen retrieval was performed in 0.1M sodium citrate buffer and detected using Diaminobenzidine (DAB)