Human CD44 antibody

CD44 is a broadly distributed transmembrane glycoprotein that plays a critical role in a variety of cellular behaviors, including adhesion, migration, invasion, and survival. CD44 mediates cell-cell and cell-matrix interactions in a large part through its affinity for hyaluronan (HA), a glycosaminoglycan constituent of extracellular matrices, but also potentially through its affinity for other ligands such as osteopontin, collagens, and matrix metalloproteinases (MMPs) . Variants of CD44 are expressed in tissues during development, including embryonic epithelia. known functions of CD44 are cellular adhesion (aggregation and migration), hyaluronate degradation, lymphocyte activation, lymph node homing, myelopoiesis and lymphopoiesis, angiogenesis, and release of cytokines. The functions of CD44 are principally dependant on cellular adhesion in one setting or another.

Product Specifications

Product Name Alternative

CD44 antigen, CDw44, Epican, Extracellular matrix receptor III, ECMR-III, GP90 lymphocyte homing/adhesion receptor, HUTCH-I, Heparan sulfate proteoglycan, Hermes antigen, Hyaluronate receptor, Phagocytic glycoprotein 1, PGP-1, Phagocytic glycoprotein I, PGP-I, LHR, MDU2, MDU3, MIC4

Host

Mouse

Antigen Species

Human

Reactivity

Human

Immunogen

Recombinant human CD44 (21-145aa) purified from E. coli

Clonality

Monoclonal

Isotype

IgG2b κ

Clone

5C10

Conjugation

Unconjugated

Applications

ELISA, WB, ICC/IF, FACS

Purification Method

By protein-G affinity chromatography

Concentration

1 mg/mL (determined by BCA assay)

Additionnal Information

CD44, ACD0826-10 µg, ACD0826-20 µg, ACD0826-50 µg, ACD0826-100 µg, ACD0826-250 µg, ACD0826-500 µg, ACD0826-1 mg, ACD0826-10, ACD0826-20, ACD0826-50, ACD0826-100, ACD0826-250, ACD0826-500, ACD0826-1

References & Citations

Cichy, J. and Pure, E. (2003) J.Cell Biol. 161, 839-843.; ; Sneath RJ, Mangham DC (1998) Mol Pathol. 51 (4) :191-200.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Applications Notes

The antibody has been tested by ELISA, Western blot, ICC/IF and FACS nalysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Scientific Category

Immunology

NCBI Accession Number

NP_001001390

Uniprot Accession Number

P16070

WB Description

The recombinant protein (200ng) was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human CD44 antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: Recombinant human CD44 protein

FACS Description

Flow cytometry analysis of CD44 in HeLa cells. The cell was stained with ACD0826 at 2-5ug for 1x10^6cells (red) . A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray) . Flow cytometry analysis of CD44 in PBMC cells. The cell was stained with ACD0826 at 2-5ug for 1x10^6cells (red) . A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray) . {ACD0826-FC2.jpg}

IF Description

ICC/IF analysis of CD44 in HeLa cells. The cell was stained with ACD0826 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .