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Mouse IL-7 Protein, His tag (Animal-Free)

Mouse IL-7 Protein, His tag (Animal-Free), expressed in E. coli

Product Specifications

Background

Interleukin-7 (IL-7) is encoded by the IL7 gene in mouse and secreted by stromal cells in the red marrow and thymus. The protein signals through the IL-7 receptor, which is a heterodimer consisting of IL-7 receptor alpha and IL-2 receptor gamma chain. IL-7 stimulates the differentiation of hematopoietic stem cells into lymphoid progenitor cells and it can stimulate proliferation of B cells, T cells and NK cells. Murine IL-7 has approximately 65% and 88% amino acid sequence identity with human and rat IL-7 and both proteins exhibit cross-species activity. IL-7 as an immunotherapy agent has been examined in many human clinical trials for various malignancies and during HIV infection

Product Name Alternative

Lymphopoietin 1 (LP-1), pre-B-cell factor, hlb368, A630026I06Rik

Accession Number

P10168

Tag

IL-7

Type

Cytokines and proteins

Applications

His tag

Purity

> 98% as determined by SDS-PAGE.

Activity

Measured in a cell proliferation assay using PHA-activated human peripheral blood lymphocytes (PBMC) .The ED₅₀ for this effect is <0.2 ng/mL.The specific activity of recombinant mouse IL-7 is > 5 x 10⁶ IU/mg.

Molecular Weight

15.8 kDa

Precautions

The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Features & Benefits

Endotoxin: <0.1 EU per 1 μg of the protein by the LAL method.

Shipping Conditions

The product is shipped with blue ice.

Storage Conditions

Lyophilized protein product should be stored desiccated below -18°C. Upon reconstitution, the protein should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (5% HSA , 10%FBS or 0.1%BSA) . Please prevent freeze-thaw cycles

Protein Length

The recombinant Mouse IL-7 Protein consists of 129 amino acids and predicts a molecular mass of 15.8 kDa

Formulation

The protein was lyophilized from sterile PBS, pH 7.4. If you have any concerns or special requirements, please confirm with us.

Applications Notes

Mouse

Other Product Names

Mouse IL-7 Protein, His tag (Animal-Free), IL-6, IFN-β2, B-Cell Differentiation Factor (BCDF), BSF-2, HPGF, HSF, MGI-2

Recommended Usage

Always centrifuge tubes before opening. It is recommended to reconstitute the lyophilized recombinant protein to a concentration of 0.1-1 mg/mL using the buffer we provided, and keep at room temperature for at least 20 min to fully dissolve. Please avoid vortex vigorously.

Preparation

E. coli

Accession Number URL

https://www.uniprot.org/uniprot/P05231

AA Sequence

Amino acid sequence derived from Mouse IL-7 (Met 26-Asn 154) (P10168) was expressed with 6xHis tag and a Met at the C-terminus

Available Sizes

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