Chk2 (Phospho Thr432) rabbit pAb

Catalytic activity:ATP + a protein = ADP + a phosphoprotein., cofactor:Magnesium., disease:Defects in CHEK2 are associated with Li-Fraumeni syndrome 2 (LFS2) [MIM:609265]; a highly penetrant familial cancer phenotype usually associated with inherited mutations in p53/TP53., disease:Defects in CHEK2 are found in some patients with osteosarcoma (OSRC) [MIM:259500]., disease:Defects in CHEK2 are found in some patients with prostate cancer (CaP) [MIM:176807]., enzyme regulation:Rapidly phosphorylated on Thr-68 by MLTK in response to DNA damage and to replication block. Kinase activity is also up-regulated by autophosphorylation., function:Regulates cell cycle checkpoints and apoptosis in response to DNA damage, particularly to DNA double-strand breaks. Inhibits CDC25C phosphatase by phosphorylation on 'Ser-216', preventing the entry into mitosis. May also play a role in meiosis. Regulates the TP53 tumor suppressor through phosphorylation at 'Thr-18' and 'Ser-20'., similarity:Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CHK2 subfamily., similarity:Contains 1 FHA domain., similarity:Contains 1 protein kinase domain., subcellular location:Isoform 10 is present throughout the cell., tissue specificity:High expression is found in testis, spleen, colon and peripheral blood leukocytes. Low expression is found in other tissues.