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His-Tag Monoclonal Antibody (4E6)

Product Specifications

Background

A polyhistidine-tag is an amino acid motif in proteins that consists of at least five histidine (His) residues, often at the N- or C-terminus of the protein. It is also known as hexa histidine-tag, 6xHis-tag, and by the trademarked name His-tag. Polyhistidine-tags are often used for affinity purification of polyhistidine-tagged recombinant proteins expressed in Escherichia coli and other prokaryotic expression systems.

Cross Reactivity

Species independent

Clonality

Monoclonal

Source

Mouse

Applications

WB; IP; IF/ICC

Dilution

WB: 1:3000 IP: 1:200 IF: 1:1000

Buffer

PBS, pH 7.4, containing 0.5%BSA, 0.02% sodium azide as Preservative and 50% Glycerol.

Storage Conditions

-20°C/1 year

Available Sizes

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