Stat1 Antibody

Product Specifications

Background

The stat proteins function both as cytoplasmic signal transducers and as activators of transcription. Stat1 is expressed as two variants of 84 and 91 kDa. Stat1 proteins contain SH2 and SH3 domains, and are components of the interferon-stimulated gene factor 3 (ISGF3) complex. This complex is the primary transcription activator induced by the binding of interferon to its receptors. In response to activation by various cytokines and growth factors, stat1 subunits become phosphorylated at tyrosine 701. This leads to translocation of stat1 to the nucleus, resulting in formation of an active ISGF3 complex. Active ISGF3 modulates the transcription of the interferon-stimulated genes. Thus, phosphorylation of Tyr-701 is critical for gene expression mediated by various cytokines and growth factors.

Synonyms

Stat1a, Stat1b, ISGF3, Stat1alpha, Stat1beta

Swiss Prot

P42224

Host

Mouse

Cross Reactivity

Human, Mouse, Rat

Target

Stat1

Clonality

Monoclonal

Isotype

IgG1

Clone

M249

Conjugation

Unconjugated

Source

Clone (M249) was generated from a recombinant protein from human Stat1. This sequence has high homology to the conserved region in rat and mouse stat1 a and b.

Applications

WB, IP

Purification

Purified by Protein A.

Dilution

WB (1:300-5000), IP (1-2ug)

Buffer

PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol

Modification

Unmodified

Storage Conditions

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.